Biotechnology Bulletin ›› 2017, Vol. 33 ›› Issue (10): 163-168.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0076

• Research report • Previous Articles     Next Articles

Isolation and Identification of an Imazethapyr-degrading Bacterium,and Optimization of Biodegradation Conditions

Lü Fan-yang1, XU Ze-hua2, MAO Xiao-jie1, SUN Jian-guang1   

  1. 1. Key Laboratory of Microbial Resources,Ministry of Agriculture / Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081
    2. Germplasm Resources Institute,Ningxia Academy of Agriculture and forestry Sciences,Yinchuan 750002
  • Received:2017-02-13 Online:2017-10-29 Published:2017-10-29
  • Contact: 孙建光,男,博士后,研究员,研究方向:农业微生物资源,环境污染修复;E-mail:jgsun@caas.ac.cn

Abstract: This work aims to isolate and identify the strain IM9603 degrading herbicide imazethapyr,and optimize the degradation condition. IM9603 was identified as Brevibacterium sp. based on morphological,physio-biochemical characteristics,and analysis of 16S rDNA. Response surface methodology Box-Behnken was applied to optimize the degradation conditions. As results,strain IM9603 degraded 93.08% of the initial 50 mg/L supply of imazethapyr under the optimal condition(pH5 and 26.2℃)within 7 days,which was its best degradability. The isolated imazethapyr-degrading strain was Brevibacterium epidermidis,and presented high degradability to imazethapyr,suggesting that is owns a great potential in the environmental rehabilitation caused by imazethapyr.

Key words: imazethapyr, biodegradation, Brevibacterium sp., response surface methodology