Enzyme Activities and Microbial Abundance of Rhizosphere Surrounding Different Tree Species in Daqing Mountain

doi:10.13560/j.cnki.biotech.bull.1985.2017-0296

Abstract

Abstract: This study aims to find the correlation between soil microbial abundance and enzyme activities of rhizosphere around different tree species in forest of the Daqing Mountain and its key factors. Real-time qPCR was used to study the variations of soil microbial abundance（bacteria and fungi）around the rhizosphere of different species，and analyzed the changing trend of three soil enzyme activities（β-glucosidase（βG），N-acetyl glucosidase（NAG）and peroxidase（Pod）and soil physicochemical properties. Principal component analysis（PCA）and Pearson correlation analysis were applied to investigate the impacts of soil physicochemical factors on the soil microbial abundance. As results，soil physicochemical properties and microbial bacteria and fungi community abundances had significant increases from spring to autumn，such as organic carbon（TOC），total nitrogen（TN），microbial biomass carbon（WC）and nitrogen（WD）as well as bacteria and fungi abundances，etc. The forest soil βG enzymes and Pod activity in Daqing Mountain was higher in summer，while NAG enzyme activity was higher in the fall. Pearson correlation analysis showed that soil microbial abundance and soil enzyme activities of different tree species in forest in the Daqing Mountain had obvious correlation，significantly positive correlation with βG enzymes activity（P < 0.05），very significantly negative correlation with Pod activity（P < 0.05），and very significantly negative correlation with βG enzyme activity（P < 0.05）. Soil physicochemical factors（TOC，TN and WC and WD）and soil microbial abundance had significantly positive correlation. It was inferred from principal component analysis，that TOC，TN，WC and WD，bacterial and fungal community abundance，and NAG can be used as the important influencing factors of the rhizosphere soil nutrient characteristics surrounding different tree species. The different tree species in Daqing Mountain forest ecosystem had profound influences on soil physicochemical index，soil microbial abundance and soil enzyme activities，and presented the strong responses with the season changing. Physicochemical index and soil microbial community abundance are the main ecological factors regulating the microbial community of rhizosphere soil in the forest and vegetation of Daqing Mountain.

Key words: Daqing Mountain, forest, soil enzyme activities, microbial community abundance

ZHANG Xing, LI Min, YAO Qing-zhi, TIE Ying, WEI Jie, CHEN Li-xia. Enzyme Activities and Microbial Abundance of Rhizosphere Surrounding Different Tree Species in Daqing Mountain[J]. Biotechnology Bulletin, 2017, 33(10): 209-216.

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