Isolation and Identification of a Secretory Siderophore Fungus,and Its Biological Activity

doi:10.13560/j.cnki.biotech.bull.1985.2021-0394

Abstract

Abstract:

A secretory siderophore producing fungus was isolated from the soil environment of Ailao Mountain primeval forest in Yunnan National Nature Reserve,and its effects on the physiological and biochemical functions of soil microorganisms infected with tobacco bacterial wilt were studied,which provides a theoretical basis for the development and utilization of the strain. Dilution coating method was used to isolate and purify the fungus producing the secretory siderophore from soil sample,the taxonomic status of the strain was identified by morphology and rDNA-ITS gene sequence. Chromazurol S（CAS）solution detection combined with full band ultraviolet absorption method was to determine the chemical structure type and activity of siderophore. DNS colorimetric method and Biolog-ECO analysis method were used to analyze the effects of strain secondary metabolites on the enzyme activity and microbial metabolism of tobacco bacterial wilt soil. The secretory siderophore-producing fungus was identified as Aspergillus welwitschiae based on morphological observations and sequence alignment analysis;the chemical structure type of the secretory siderophore of the strain was carboxylates,and its siderophore activity was 78.79%. The secondary metabolites of the strain significantly increased the activities of soil invertase and urease（P<0.05）by 13.7% and 14.46% respectively. The secondary metabolites of the strain also significantly improved the carbon source utilization capacity：Average color change rate（AWCD）and diversity index（Shannon index and richness index）increased by 86.15%,50.43% and 66.67% respectively. In conclusion,siderophore microbial resources are stored in primeval forest soil environment. The improvement of physiological and biochemical functions of the soil microbial community mediated by strain-secreted siderophore is an important technical means to optimize the management of the soil ecosystem.

Key words: soil in primeval forest, siderophore, Ralstonia solanaceous, soil enzyme, Biolog-ECO

ZOU Xue-feng, LI Ming-gang, BAO Ling-feng, CHEN Qi-bin, ZHAO Jiang-yuan, WANG Lin, PU Yong-yu, HAO Da-cheng, ZHANG Qing, YANG Pei-wen. Isolation and Identification of a Secretory Siderophore Fungus,and Its Biological Activity[J]. Biotechnology Bulletin, 2022, 38(3): 130-138.

Figures/Tables 8

Fig. 1 Plate culture of the strain（PDA and CAS plate） A：1-5F1 strain. B：1-5F1 strain was inoculated in double plate culture（The lower layer was water agar medium supplemented with chromeazurol S（CAS）,and the upper layer was solid medium without iron）

Fig.2 Absorbance values of strain 1-5F1 siderophores in full band

Fig. 3 Microscopic morphologies of conidia of the strain A：10 000 times. B：2 500 times

Fig. 4 Phylogenetic tree of strain 1-5F1 based on ITS gene sequence The phylogenetic evolutionary tree is constructed by the adjacent method,and the self-propagating number was 1 000. The values at each of the nodes refer to the contribution values of the self-expanding number. The number in parenthesis after each strain name is ITS gene sequence entry number in GenBank

Fig.5 Effects of metabolites of 1-5F1 strain on the activities of sucrase（S-SC）and urease（S-UE）in the rhiz-osphere soil of tobacco bacterial wilt

Fig.6 Mean color change rate（AWCD）of tobacco bacte-rial wilt rhizosphere soil treated by the metabolites of 1-5F1 strain with time

Fig.7 Differences in carbon source utilization abilities of soil microbial communities treated with different concentrations of metabolites of bacterial strains

Fig.8 Changes in soil microbial functional diversity index with time under different concentrations of metabolites from the strain

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