PCR-based Method for the Rapid Detection of ACC Deaminase-producing Rhizosphere Bacteria

doi:10.13560/j.cnki.biotech.bull.1985.2017-0370

Abstract

Abstract: Producing ACC（1-aminocyclopropane-1-carboxylate）deaminase to reduce ethylene levels and eliminate salt stress is the key mechanism of plant-growth-promoting rhizobacteria（PGPR）in improving plant growth and stress tolerance. This work aims to establish an expeditious approach to screen bacteria containing ACC deaminase based on polymerase chain reaction（PCR）. We set the acdS gene encoding the ACC deaminase as the probe,then selected three pairs of primers（acdSf3/acdSr4,DegACCf/DegACCr and F1936f/F1938r）respectively,and detected the various bacterial strains isolated from the root and rhizosphere soil of diverse halophytes and Populus deltoids. The results showed that single specific amplified bands were produced and the rate of amplification was high when using the primers acdSf3/acdSr4 combined with touch-down PCR methods;however,DegACCf/DegACCr or F1936f/F1938r was in poor specificity. Finally,we acquired 25 strains containing acds gene from 257 bacterial isolates,aiming at laying a foundation for reserving rich and functional microbial resources and further studying the genetic diversity and function of acdS in PGPR.

Key words: halophytes, poplar, plant growth promoting rhizobacteria, endophytic bacteria, ACC deaminase-producing bacterium

QIN Yuan, PAN Xue-yu, YUAN Zhi-lin. PCR-based Method for the Rapid Detection of ACC Deaminase-producing Rhizosphere Bacteria[J]. Biotechnology Bulletin, 2017, 33(11): 112-122.

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