Study On the Degradation and Mechanism of Phenolic Compounds by the Thauera sp. K11

doi:10.13560/j.cnki.biotech.bull.1985.2017-0452

Abstract

Abstract: The objective of this work is to analyze the degradation and pathways of a Thauera sp. K11 in genus Thauera to phenolic compounds in phenolic wastewater. Thauera sp. K11 was isolated from petrochemical wastewater treatment plant .The 16S rRNA gene and key enzyme gene were cloned and analyzed and phylogenetic analysis was carried out. The mechanism of phenol degradation was explored at the gene level. The degradation efficiency and mechanism of phenolic compounds were studied by gas chromatography. The 16S rRNA phylogenetic analysis revealed that the bacterium Thauera sp. K11 was a species of bacteria from the genus Thauera，which degraded 11 phenolic compounds. The degradation rates of the five phenolic compounds were > 90 % in 72 h. The K11 phenol hydroxylase and catechol dioxygenase genes were cloned，and the enzyme activity assay showed that K11 catalyzed the conversion of phenol to catechol by phenol hydroxylase and then catalyzed the production of 2-HMSA with catechol-2，3-dioxygenase. In conclusion，the bacterium Thauera sp. K11 is a species of Thauera bacterium capable of degrading a variety of phenolic compounds，and possesses the strong capability of degrading phenolic pollutants；the degradation pathway is by phenol→catechol→2-HMSA.

Key words: genus Thauera, petrochemical wastewater, phenolic compounds, biodegradation, phenol hydroxylase, catechol-2, 3-dioxygenase

QIAO Neng-hu, ZHANG Jing-jing, LIU De-jian, TANG Xiao-sheng, XI Li-jun, LIU Jian-guo. Study On the Degradation and Mechanism of Phenolic Compounds by the Thauera sp. K11[J]. Biotechnology Bulletin, 2017, 33(10): 184-190.

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