Research Progress on Duplex-specific Nuclease Mediated Biosensors

doi:10.13560/j.cnki.biotech.bull.1985.2017-0786

Abstract

Abstract: Duplex-specific nuclease（DSN）is a kind of protein enzyme that can effectively identify and cleave double-stranded DNA or DNA strand of hybridized DNA/RNA complex,but not affect the single strand DNA and single/double strand RNA. Thus,the DSN has been widely used in the areas of biology and medicine. Based on the property of DSN specifically cleaving DNA and RNA and equipped with signal output and different amplification,a series of biological sensing technology,such as colorimetric method,fluorescent method,electrochemical method,etc.,have been established. By these methods a series of biomarkers and food safety risk factors,like miRNAs,mRNA and heavy metal ions,may be detected. In addition,DSN-mediated biosensors with emerging nanomaterial also display a great advantage in analyzing the RNA and other material sensing. Firstly,we present an overview of the basic knowledge of DSNs,including the structure,properties and cleaving ways. Secondly,we give a classified overview of DSN-mediated biosensor based on the various readout signals,like optical sensors,electrochemical sensors and magnetic sensors,etc. The detailed contents covered the principles of varied sensors,and the detection ranges and sensitivity of each method;meanwhile,the types of all the targets that can be detected by DSN-mediated biosensors are summarized,expecting that this is conducive to further deeply apply DSN. Finally,the disadvantages of the DSN-mediated biosensors are summarized and the future development trend is prospected,aimed at guiding people to avoid relevant problems in the future when they use the DSN-mediated biosensors,and to develop faster,more convenient and more sensitive biosensors.

Key words: duplex-specific nuclease, colorimetric, fluorescence, electrochemical, biosensor

XIAO Xing-ning, ZHU Long-jiao, LI Xiang-yang, LUO Yun-bo, XU Wen-tao. Research Progress on Duplex-specific Nuclease Mediated Biosensors[J]. Biotechnology Bulletin, 2018, 34(9): 39-47.

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