Research Advances on RNAi Mechanism and Its Application

doi:10.13560/j.cnki.biotech.bull.1985.2017.05.001

Abstract

Abstract: RNA interference（RNAi）is a gene silencing phenomenon induced by small non-coding RNA（sncRNA）,which is widely found in eukaryotes. RNAi has become the focus of biological research with some achievements since its discovery. In order to provide some references for the further study of RNAi,the mechanism of RNAi,the key factors involved in RNAi and the application of RNAi are reviewed in this paper.

Key words: RNAi, mechanism, dicer, argonaute protein family, RdRP

FENG Xiao-yan ZHANG Shu-zhen. Research Advances on RNAi Mechanism and Its Application[J]. Biotechnology Bulletin, 2017, 33(5): 1-8.

References

[1] Nejepinska J, Flemr M, Svoboda P. The Canonical RNA Interference Pathway in Animals[M]// Mallick B, Ghosh Z. Regulatory RNAs-Basics, Methods and Applications. Berlin：Springer, 2012：111-149.
[2] 崔喜艳, 孙小杰, 刘忠野, 等. RNAi机制及在植物中应用的研究概述[J]. 吉林农业大学学报, 2013, 35（2）：160-166.
[3] Czech B, Hannon GJ. Small RNA sorting：matchmaking for Argonautes[J]. Nature Reviews Genetics, 2010, 12（1）：19-31.
[4] Fire A, Xu S, Montgomery MK, et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans[J]. Nature, 1998, 391（6669）：806-811.
[5] Brantl S. Antisense-RNA regulation and RNA interference[J]. Biochimica et Biophysica Acta, 2002, 1575（1-3）：15-25.
[6] Cerutti H, Casas-Mollano JA. On the origin and functions of RNA-mediated silencing：from protists to man[J]. Current Genetics, 2006, 50（2）：81-99.
[7] Torres-Martínez S, Ruiz-Vázquez RM. RNAi pathways in Mucor：A tale of proteins, small RNAs and functional diversity[J]. Fungal Genetics and Biology, 2015, 90：44-52.
[8] 李绍祥, 郭汉斌, 曹建彪. RNA干扰的途径和机制[J]. 昆明医科大学学报, 2012, 33（11）：148-151.
[9] Jinek M, Doudna JA. A three-dimensional view of the molecular machinery of RNA interference[J]. Nature, 2009, 457（7228）：405-412.
[10] Pereira P, Queiroz JA, Figueiras A, et al. Affinity approaches in RNAi-based therapeutics purification[J]. Journal of Chromatography B, 2016, 1021（10）：45-56.
[11] 哀建国, 杨勇. RNA介导的植物基因沉默作用及其应用[J]. 浙江林学院学报, 2005, 22（1）：123-128.
[12] Ramachandran PV, Ignacimuthu S. RNA Interference—A Silent but an Efficient Therapeutic Tool[J]. Applied Biochemistry and Biotechnology, 2013, 169（6）：1774-1789.
[13] Nykanen A, Haley B, Zamore PD. ATP requirements and small interfering RNA structure in the RNA interference pathway[J]. Cell, 2001, 107（3）：309-321.
[14] Schaffer AE, Eggens VRC, Caglayan AO, et al. CLP1 founder mutation links tRNA splicing and maturation to cerebellar development and neurodegeneration[J]. Cell, 2014, 157（3）：651-663.
[15] Ku SH, Jo SD, Lee YK, et al. Chemical and structural modifications of RNAi therapeutics[J]. Advanced Drug Delivery Reviews, 2015, 78（3）：250-258.
[16] Hannon GJ. RNA interference[J]. Nature, 2002, 418（6894）：244-251.
[17] Gish RG, Yuen M, Chan HLY, et al. Synthetic RNAi triggers and their use in chronic hepatitis B therapies with curative intent[J]. Antiviral Research, 2015, 121：97-108.
[18] Liu J, Carmell MA, Rivas FV, et al. Argonaute2 is the catalytic engine of mammalian RNAi[J]. Science, 2004, 305（5689）：1437-1441.
[19] Haley B, Zamore PD. Kinetic analysis of the RNAi enzyme comp-lex[J]. Nature Structural & Molecular Biology, 2004, 11（7）：599-606.
[20] Liu Y, Tan H, Tian H, et al. Autoantigen La promotes efficient RNAi, antiviral response, and transposon silencing by facilitating multiple-turnover RISC catalysis[J]. Molecular Cell, 2011, 44 （3）：502-508.
[21] 赵雅, 吴立刚. 基因表达调控多面手——microRNA和siRNA的作用机制[J]. 生命科学, 2010, 22（7）：628-633.
[22] Petersen CP, Bordeleau M, Pelletier J, et al. Short RNAs repress translation after initiation in mammalian cells[J]. Molecular Cell, 2006, 21（4）：533-542.
[23] Wu L, Fan J, Belasco JG. Importance of translation and nonnucleolytic ago proteins for on-target RNA interference[J]. Current Biology, 2008, 18（17）：1327-1332.
[24] Kim DH, Villeneuve LM, Morris KV, et al. Argonaute-1 directs siRNA-mediated transcriptional gene silencing in human cells[J]. Nature Structural & Molecular Biology, 2006, 13（9）：793-797.
[25] 史毅, 金由辛. RNA干扰与siRNA（小干扰RNA）研究进展 [J]. 生命科学, 2008, 20（2）：196-201.
[26] Morris KV, Chan SW, Jacobsen SE, et al. Small interfering RNA-induced transcriptional gene silencing in human cells[J]. Science, 2004, 305（5688）：1289-1292.
[27] Sherman JH, Munyikwa T, Chan SY, et al. RNAi technologies in agricultural biotechnology：The Toxicology Forum 40th Annual Summer Meeting[J]. Regulatory Toxicology and Pharmacology, 2015, 73（2）：671-680.
[28] Zhang C, Ruvkun G. New insights into siRNA amplification and RNAi[J]. RNA Biology, 2012, 9（8）：1045-1049.
[29] 和琼姬, 燕飞, 陈剑平. RNA干扰机制及其主要蛋白因子研究进展[J]. 浙江农业学报, 2011, 23（2）：415-420.
[30] Kim VN, Han J, Siomi MC. Biogenesis of small RNAs in animals [J]. Nature Reviews Molecular Cell Biology, 2009, 10（2）：126-139.
[31] 何苗, 王子卫. miRNA与siRNA胃癌相关研究的现状及进展[J]. 遗传, 2011, 33（9）：925-930.
[32] 唐彬, 肖斌, 邹全明, 等. Mirtron——miRNA合成的新途径 [J]. 生命的化学, 2009, 29（3）：315-317.
[33] Yang J, Lai EC. Alternative miRNA biogenesis pathways and the interpretation of core miRNA pathway mutants[J]. Molecular Cell, 2011, 43（6）：892-903.
[34] Sarett SM, Nelson CE, Duvall CL. Technologies for controlled, local delivery of siRNA[J]. Journal of Controlled Release, 2015, 218：94-113.
[35] 张超, 庞全海. siRNA与miRNA在生物体基因调控中沉默机制的比较[J]. 中国生物化学与分子生物学报, 2012, 28（5）：393-398.
[36] Huntzinger E, Izaurralde E. Gene silencing by microRNAs：contributions of translational repression and mRNA decay[J]. Nature Reviews Genetics, 2011, 12（2）：99-110.
[37] Humphreys DT, Westman BJ, Martin DI, et al. MicroRNAs control translation initiation by inhibiting eukaryotic initiation factor 4E/cap and poly（A）tail function[J]. Proceedings of the National Academy of Sciences, 2005, 102（47）：16961-16966.
[38] Piao X, Zhang X, Wu L, et al. CCR4-NOT deadenylates mRNA associated with RNA-induced silencing complexes in human cells[J]. Molecular and Cellular Biology, 2010, 30（6）：1486-1494.
[39] 吴丹, 胡兰. miRNA及其研究进展[J]. 中国畜牧兽医, 2006, 33（1）：40-42.
[40] Jover-Gil S, Candela H, Ponce MR. Plant microRNAs and development[J]. International Journal of Developmental Biology, 2005, 49（5-6）：733-744.
[41] 廖珂, 柴志欣, 钟金城. 小分子非编码RNA与雄性不育[J]. 生物技术通报, 2016, 32（1）：33-40.
[42] Aravin AA, Hannon GJ, Julius B. The Piwi-piRNA pathway provides an adaptive defense in the transposon arms race[J]. Science, 2007, 318（5851）：761-764.
[43] Ketting RF. The Many Faces of RNAi[J]. Developmental Cell, 2011, 20（2）：148-161.
[44] Siomi MC, Sato K, Pezic D, et al. PIWI-interacting small RNAs：the vanguard of genome defence[J]. Nature Reviews Molecular Cell Biology, 2011, 12（4）：246-258.
[45] Nishida KM, Saito K, Mori T, et al. Gene silencing mechanisms mediated by Aubergine-piRNA complexes in Drosophila male gonad[J]. RNA, 2007, 13（11）：1911-1922.
[46] Brent BT, Findley SD, Ling J, et al. Drosophila PIWI associates with chromatin and interacts directly with HP1a[J]. Genes & Development, 2007, 21（18）：2300-2311.
[47] 袁婺洲, 吴秀山. RNAi机制研究的最新进展[J]. 生命科学研究, 2003, 7（1）：8-14.
[48] Höck J, Meister G. The Argonaute protein family[J]. Genome Biology, 2008, 9（2）：1-8.
[49] Bak CW, Yoon TK, Choi Y. Functions of PIWI proteins in spermatogenesis[J]. Clinical & Experimental Reproductive Medicine, 2011, 38（2）：61-67.
[50] Seto AG, Kingston RE, Lau NC. The Coming of Age for Piwi Proteins[J]. Molecular Cell, 2007, 26（5）：603-609.
[51] Zhou X, Liao Z, Jia Q, et al. Identification and characterization of Piwi subfamily in insects[J]. Biochemical & Biophysical Research Communications, 2007, 362（1）：126-131.
[52] Pak J, Maniar JM, Mello CC, et al. Protection from Feed-Forward Amplification in an Amplified RNAi Mechanism[J]. Cell, 2012, 151（4）：885-899.
[53] Witold F, Bhattacharyya SN, Nahum S. Mechanisms of post-transcriptional regulation by microRNAs：are the answers in sight?[J]. Nature Reviews Genetics, 2008, 9（2）：102-114.
[54] Concetta L, Paterson BM. Identification of an RNA-dependent RNA polymerase in Drosophila involved in RNAi and transposon suppression[J]. Proceedings of the National Academy of Sciences, 2009, 106（37）：15645-15650.
[55] 马丽, 张春庆. RNA干扰机制及应用研究进展[J]. 北方园艺, 2012（10）：191-193.
[56] Kimber MJ, Mckinney S, Mcmaster S, et al. flp gene disruption in a parasitic nematode reveals motor dysfunction and unusual neuronal sensitivity to RNA interference[J]. Faseb Journal, 2007, 21（4）：1233-1243.
[57] Zhang JZ, Liu XJ, Zhang JQ, et al. Silencing of two alternative splicing-derived mRNA variants of chitin synthase 1 gene by RNAi is lethal to the oriental migratory locust, Locusta migratoria manilensis（Meyen）[J]. Insect Biochemistry & Molecular Biology, 2010, 40（11）：824-833.
[58] Dong TT, Hu ZL, Deng L, et al. A tomato MADS-box transcription factor, SlMADS1, acts as a negative regulator of fruit ripening[J]. Plant Physiology, 2013, 163（2）：1026-1036.
[59] 李方华, 侯玲玲, 苏晓华, 等. RNA干扰的研究进展及应用 [J]. 生物技术通讯, 2010, 21（5）：740-745.
[60] Clemens JC, Worby CA, Simonson-Leff N, et al. Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways[J]. Proceedings of the National Academy of Sciences, 2000, 97（12）：6499-6503.
[61] Judge AD, Marjorie R, Iran T, et al. Confirming the RNAi-mediated mechanism of action of siRNA-based cancer therapeutics in mice[J]. Journal of Clinical Investigation, 2009, 119（3）：661-673.
[62] Brandt MRG, Kirste AG, Pozzuto T, et al. Adenovirus vector-mediated RNA interference for the inhibition of human parvovirus B19 replication[J]. Virus Research, 2013, 176（1-2）：155-160.
[63] 王健, 邹雯. 中医药治疗艾滋病的研究进展[J]. 中国艾滋病性病, 2014, 20（10）：61-63.
[64] Brake OT, Legrand N, Eije KJV, et al. Evaluation of safety and efficacy of RNAi against HIV-1 in the human immune system（Rag-2 -/- γ c -/- ）mouse model[J]. Gene Therapy, 2008, 16（1）：148-153.
[65] Das AT, Brummelkamp TR, Westerhout EM, et al. Human immunodeficiency virus type 1 escapes from RNA interference-mediated inhibition[J]. Journal of Virology, 2004, 78（5）：2601-2605.
[66] 孔怡淳, 赵堪兴. RNA干涉技术及眼科应用[J]. 中华实验眼科杂志, 2014, 32（4）：370-374.
[67] Zheng YP, Liu H, Zeng H, et al. Downregulation of lentivirus-mediated ILK RNAi on tractional force generation in human retinal Müller cells[J]. Acta Pharmacologica Sinica, 2009, 30（12）：1625-1633.
[68] 尚仁福, 吴立刚. RNA干扰的机制及其应用[J]. 生命科学, 2016, 28（5）：576-583.