Transcriptome Sequencing of Stamen in Muskmelon Male Sterile Lines at Different Developmental Stages

doi:10.13560/j.cnki.biotech.bull.1985.2018-0306

Abstract

Abstract: In order to explore the mechanism of male sterility in muskmelon,the correlation between pollen abortion and differentially-expressed genes at different stages of male sterility and stamen development was studied at the molecular level. The transcriptome sequencing of bud（while its diameter was 2 mm and 5 mm）stamen from male sterile（MS）line of muskmelon was conducted,differentially-expressed genes were screened,and differentially-expressed genes were analyzed by fluorescent quantitative method. Totally 224 differentially-expressed genes were identified. GO function analysis of them showed that all the genes belonged to 3 branches of biosynthesis,molecular function and cellular components. Among them,9 differentially-expressed genes were related to various enzymes,2 related to carboxylesterase homologous,4 related to carbohydrate enzymes synthesis,and 1 gene related to signal transduction and 1 gene related to zeaxanthin biosynthesis,respectively. qRT-PCR verification revealed that the expression levels of carbohydrate-related enzymes and zeaxanthin biosynthesis-related genes in 2 mm buds and stamens were lower than those in 5 mm buds and stamens. The expressions of cell signal transduction-related genes in MS line 2 mm stamens were higher than that of 5 mm stamens. The above results indicate that there is a certain correlation between the occurrence of male sterility and the increase of energy metabolism-related gene expression and the reduction of the expression of genes related to cell signaling.

Key words: muskmelon, male sterility, transcriptome, differentially gene

DAI Dong-yang, YUAN Li-wei, SHENG Yun-yan, ZHENG Qun. Transcriptome Sequencing of Stamen in Muskmelon Male Sterile Lines at Different Developmental Stages[J]. Biotechnology Bulletin, 2018, 34(8): 93-100.

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