A Rapid Detection Method of Residual Genomic DNA in Total RNA Samples from Lilium regale

doi:10.13560/j.cnki.biotech.bull.1985.2018-0364

Abstract

Abstract: The residual genomic DNA in total RNA seriously affects the detection accuracy of qRT-PCR. In order to detect residual DNA in the RNA sample,a pair of primers LDRG-F：5'-CTCTTTTATGACGAGGTTGGTA-3' and LDRG-R：5'-CAGGGAAATCGGTCAGTGTT-3'were designed based on partial sequence of introns in the house-keeping gene lTIP41-like. Then this pair of primers were used to detect the total RNA samples extracted by using three different RNA extraction kits and the cDNA samples obtained by using two different first strand cDNA synthesis kits via PCR amplification. The result showed that using the primers helped to rapidly detect whether or not there were the residual genomic DNA in total RNA and cDNA samples from Lilium regal.

Key words: Lilium regale, residual genomic DNA, TIP41-like, total RNA, detection

DU Wen-kai, YUAN Su-xia, HU Feng-rong. A Rapid Detection Method of Residual Genomic DNA in Total RNA Samples from Lilium regale[J]. Biotechnology Bulletin, 2018, 34(10): 81-86.

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