Isolation,Identification,and Antibacterial Activity of Fungi Associated with Marine Organisms

doi:10.13560/j.cnki.biotech.bull.1985.2018-0856

Abstract

Abstract: Isolation,identification and antibacterial activity analysis of fungus associated with sea grasses,sponges and stony corals from Wenchang sea area of Hainan were conducted. Three media were used to isolate and purify the fungi associated with these marine organisms,the isolated fungi were identified according to their ITS sequences,and their antibacterial activities were tested using standard disk diffusion. Total 113 strains of fungi were isolated from 10 sea grasses,9 sponges,and 11 stony corals. The 32 strains of independent fungi,belonging to 10 genera within 8 orders of Ascomycota,Basidiomycotina and Deutemycotina,were identified by ITS sequences analysis. The percentage of Ascomycota was as high as 58.82%,that of Deutemycotina was 32.35% and Basidiomycotina was 8.83%,respectively. The results of antibacterial activity showed that the fermentation products of 12 strains of fungi had strong antibacterial activity against Bacillus subtilis and Staphylococcus aureus. In sum,the diversity and antibacterial activity of fungi associated with sea grasses,sponges and stony corals from Wenchang sea area of Hainan are revealed initially.

Key words: sea grasses, sponges, stony corals, isolation, antibacterial activity

ZHANG Sheng-liang, CHU Xiao-xiao, ZHAO You-xing, KONG Fan-dong, HUANG Xiao-long. Isolation,Identification,and Antibacterial Activity of Fungi Associated with Marine Organisms[J]. Biotechnology Bulletin, 2019, 35(3): 59-64.

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