Effect of Signal Peptide-like Sequence in DrwH on Its Antioxidant Function

doi:10.13560/j.cnki.biotech.bull.1985.2018-1050

Abstract

Abstract: DrwH,a hydrophobic LEA5C protein of Deinococcus radiodurans,is involved in the process of cell tolerance to oxidative and salinity stresses. Previous studies suggest the presence of a signal peptide-like sequence of 19 amino acids in the N-terminal of DrwH protein. In order to explore the effect of the signal peptide-like sequence on the antioxidant function of DrwH,DrwH protein was truncated to different fragments and then recombinant Escherichia coli strains containing the different fragments were constructed,and their growths were analyzed and compared between normal culture and oxidative stress. The results showed that overexpression of the fusion protein containing the signal peptide-like sequence not only inhibited the growth of E. coli,but also caused DrwH protein to lose its antioxidant capacity. qRT-PCR results demonstrated that,after induction by IPTG,the full-length protein DrwH containing signal peptide-like sequence and the WHy-domain were accumulated at the transcriptional level,and the proteins exerted its biological function under excessive accumulation. This indicates that the signal peptide-like sequence presents an important influence on the expression of the full-length protein DrwH and its antioxidant function.

Key words: Deinococcus radiodurans, DrwH protein, WHy domain, signal peptide-like, antioxidant function

GUO Lei-zhou, HAN Jia-hui, TANG Yin, LI Jiang, HUANG Cheng, DAI Qi-lin, WANG Jin, PING Shu-zhen, JIANG Shi-jie. Effect of Signal Peptide-like Sequence in DrwH on Its Antioxidant Function[J]. Biotechnology Bulletin, 2019, 35(5): 125-132.

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