Biotechnology Bulletin ›› 2019, Vol. 35 ›› Issue (7): 10-16.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0029

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Cloning,Expression and Bioinformatics Analyses of StSRP1 Gene in Solanum tuberosum

PANG Peng-xiang, CHANG Yan-nan, YU Rui-min, GAO Gang   

  1. Collage of Life Science,Shanxi Normal University,Linfen 041004
  • Received:2019-01-07 Online:2019-07-26 Published:2019-07-29

Abstract: The expression of stress-related genes(StSRP1)leads to the accumulation of metabolites and changes in biochemical and physiological pathways,which are essential for plants to adapt to adverse stress conditions. By studying the structure of potato StSRP1 and its expression in stress,the foundation for verifying the role of potato StSRP1 in the process of resistance to biological stress is laid. Electronic cloning was used to clone the full-length sequence of StSRP1 gene,bioinformatics was to analyze the nucleic acid sequence,amino acid sequence,protein structure and promoter of StSRP1. Based on the NCBI database,20 strains of stress-related protein sequences highly matched with StSRP1 were selected to construct a phylogenetic tree,and SRP proteins among different species were compared. StSRP1 induced by ABA,MJ and pathogen was analyzed by qRT-PCR. The full length of the cloned sequence was 867 bp,encoding 288 amino acids,no signal peptide sequence,no transmembrane region,34 possible phosphorylation sites,hydrophilic protein,and localized to the endoplasmic reticulum. In sum,the expression of StSRP1 is regulated by ABA,MJ and pathogens. StSRP1 may be involved in the potato stress response process.

Key words: potato, stress-related protein, bioinformatics, qRT-PCR