Analysis of Diversity of Malachite Green-degrading Microbial Community and Degradation Characteristics of Efficiently-degrading Bacteria

doi:10.13560/j.cnki.biotech.bull.1985.2019-0135

Abstract

Abstract:

Using triphenylmethane dye malachite green as the sole carbon source,the enriched culture degrading malachite green was obtained by continuous passage domestication. High-throughput sequencing was applied to analyze the microbial community change of the enriched culture,and the main microbial information related to degradation was acquired,and further an efficient strain degrading malachite green was isolated and purified. This strain degraded 91.2% of 50 mg/L malachite green within 7 days. The strain was initially identified as a Pseudomonas,and named as Pseudomonas veronii JW3-6.The optimized condition of degradation by this strain were temperature 30℃,pH 7,and inoculation concentration 1×10⁷ CFU/mL.

Key words: malachite green, high-throughput sequencing, microbial degradation, Pseudomonas

WANG Ya-ni, SONG Jin-long, HAN Gang, MU Ying-chun, JIANG Xu, WANG Jin-yao, RUAN Zhi-yong, LI Le. Analysis of Diversity of Malachite Green-degrading Microbial Community and Degradation Characteristics of Efficiently-degrading Bacteria[J]. Biotechnology Bulletin, 2019, 35(9): 150-155.

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