Screening of Bacillus sp. Against Vegetable Root-knot Nematode and Study on Its Biocontrol Mechanism

doi:10.13560/j.cnki.biotech.bull.1985.2021-0409

Abstract

Abstract:

Bacillus sp. are a group of important biocontrol agents for the management of plant disease. In order to search for excellent Bacillus strain for vegetable root knot nematode,62 Bacillus sp. were isolated from the suppression soil of root knot nematode. The mortality of the second stage juvenile and the inhibition of egg hatch by the fermentation filtrates of the strains were conducted to primarily screen biocontrol strains,and pot experiments were used to determine the control effects of biocontrol strains. Results demonstrated that treatments with bactreial suspensions of strains NB-04 reduced root-knot index by 61.6%. Further based on morphological characteristics and 16S rDNA gene sequence analysis,strain NB-04 was identified as Bacillus methylotrophicus. To preliminary study the biocontrol mechanism of NB-04,the influence of fermention filtrates on M. incognita egg catalase,acetylcholinesterase,carboxylesterase,and the content of egg total sugar and protein were determined. The results showed that the activity of egg catalase,acetylcholinesterase,and carboxylesterase decreased significantly,as well as the content of total sugar and protein significantly declined. This study provides the basis for the research and development of bio-nematicide.

Key words: Meloidogyne incognita, biological control, Bacillus methylotrophicus, biocontrol mechanism

ZHANG Jie, XIA Ming-cong, ZHU Wen-qian, LIANG Juan, SUN Run-hong, XU Wen, WU Chao, YANG Li-rong. Screening of Bacillus sp. Against Vegetable Root-knot Nematode and Study on Its Biocontrol Mechanism[J]. Biotechnology Bulletin, 2021, 37(7): 175-182.

Figures/Tables 8

References 29

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处理 Treatment	死亡率 Mortality/%	校正死亡率 Adjusted mortality/%	卵孵化率 Egg hatch rate/%	孵化抑制率 Inhibition rate of egg hatch/%
NB-04	94.2±1.2 a	84.9 a	13.6±1.8 g	78.6 a
NB-36	90.4±2.5 ab	75.1 ab	12.0±1.5 g	81.1 a
NB-18	89.3±1.5 abc	72.4 abc	38.4±1.2 c	39.5 fg
NB-21	89.2±1.3 abc	72.1 abc	33.7±2.1 cd	46.9 ef
NB-12	88.6±0.4 abc	70.5 abc	13.5±1.6 g	78.7 a
NB-54	86.9±0.9 abcd	66.2 abc	28.5±3.3 de	55.1 de
NB-53	86.2±2.2 abcd	64.3 abcd	22.5±1.4 ef	64.6 bcd
NB-29	86.0±0.4 abcd	63.9 abcd	45.2±3.7 b	28.8 g
NB-15	85.7±0.1 abcd	63.1 abcd	18.5±1.9 fg	70.9 abc
NB-46	85.6±2.0 abcd	62.3 abcd	27.5±3.0 de	56.7 de
NB-17	85.2±2.0 bcd	61.8 abcd	27.8±1.7 de	56.2 de
NB-45	84.6±2.2 bcd	60.2 abcd	25.0±1.7 ef	60.6 cd
NB-31	81.4±4.1 cde	52.1 bcd	14.8±2.2 g	76.7 ab
NB-26	79.6±4.0 de	47.5 bcd	35.5±2.0 c	44.1 ef
NB-33	79.3±1.2 de	46.5 cd	27.8±2.0 de	56.2 de
NB-11	75.8±3.2 e	37.4 d	15.1±0.7 g	76.2 ab
对照 Control	61.2±6.0 f	/	63.5±4.3 a	/

处理 Treatment	死亡率 Mortality/%	校正死亡率 Adjusted mortality/%	卵孵化率 Egg hatch rate/%	孵化抑制率 Inhibition rate of egg hatch/%
NB-04	94.2±1.2 a	84.9 a	13.6±1.8 g	78.6 a
NB-36	90.4±2.5 ab	75.1 ab	12.0±1.5 g	81.1 a
NB-18	89.3±1.5 abc	72.4 abc	38.4±1.2 c	39.5 fg
NB-21	89.2±1.3 abc	72.1 abc	33.7±2.1 cd	46.9 ef
NB-12	88.6±0.4 abc	70.5 abc	13.5±1.6 g	78.7 a
NB-54	86.9±0.9 abcd	66.2 abc	28.5±3.3 de	55.1 de
NB-53	86.2±2.2 abcd	64.3 abcd	22.5±1.4 ef	64.6 bcd
NB-29	86.0±0.4 abcd	63.9 abcd	45.2±3.7 b	28.8 g
NB-15	85.7±0.1 abcd	63.1 abcd	18.5±1.9 fg	70.9 abc
NB-46	85.6±2.0 abcd	62.3 abcd	27.5±3.0 de	56.7 de
NB-17	85.2±2.0 bcd	61.8 abcd	27.8±1.7 de	56.2 de
NB-45	84.6±2.2 bcd	60.2 abcd	25.0±1.7 ef	60.6 cd
NB-31	81.4±4.1 cde	52.1 bcd	14.8±2.2 g	76.7 ab
NB-26	79.6±4.0 de	47.5 bcd	35.5±2.0 c	44.1 ef
NB-33	79.3±1.2 de	46.5 cd	27.8±2.0 de	56.2 de
NB-11	75.8±3.2 e	37.4 d	15.1±0.7 g	76.2 ab
对照 Control	61.2±6.0 f	/	63.5±4.3 a	/

处理 Treatment	根结指数 Root-knot index	防治效果 Control effect/%	株高 Plant height/cm	鲜重 Fresh weight/g
NB-04	31.1±4.3 cd	61.6	52.0±4.5 a	23.9±1.0 a
NB-36	34.7±3.0 c	57.2	49.6±4.7 ab	21.5±2.9 a
NB-12	40.4±1.3 bc	50.1	46.1±3.7 ab	18.9±2.4 ab
NB-18	45.0±2.1 b	44.4	39.7±5.6 ab	16.4±2.5 ab
0.5% 阿维菌素 0.5% Avermectin	24.9±1.8 d	69.2	46.3±2.5 ab	19.8±2.0 ab
对照 Control	81.0±2.1 a	/	36.5±3.5 b	16.9±2.2 b

处理 Treatment	根结指数 Root-knot index	防治效果 Control effect/%	株高 Plant height/cm	鲜重 Fresh weight/g
NB-04	31.1±4.3 cd	61.6	52.0±4.5 a	23.9±1.0 a
NB-36	34.7±3.0 c	57.2	49.6±4.7 ab	21.5±2.9 a
NB-12	40.4±1.3 bc	50.1	46.1±3.7 ab	18.9±2.4 ab
NB-18	45.0±2.1 b	44.4	39.7±5.6 ab	16.4±2.5 ab
0.5% 阿维菌素 0.5% Avermectin	24.9±1.8 d	69.2	46.3±2.5 ab	19.8±2.0 ab
对照 Control	81.0±2.1 a	/	36.5±3.5 b	16.9±2.2 b