Difference Expression Analysis in Different Tissues of Agrotis ipsilon Reveals the Possible Mechanism of Wing Development

doi:10.13560/j.cnki.biotech.bull.1985.2019-0336

Abstract

Abstract: Black cutworm（Agrotis ipsilon）is a typical migratory agricultural pest,its wing development plays an important role in the insect migration process,and the identification and functional analysis of its specific control genes need to be further studied. The transcriptome from black cutworm whole larvae,adult head,chest,and abdomen transcriptome were sequenced and assembled. The assembled transcriptome size was 243.4 Mb and N50 contig size was 668 bp. A total of 429 288 transcripts,37 744 independent genes and 373 KEGG database annotation pathways were assembled. Wnt signaling pathways are the key ones for insect wing development. In Wnt signaling pathways in the black cutworm transcriptome,53 elements for example CaN,Frizzled and Siah-1 were annotated. CaN,Frizzled and Siah-1 in the thoracic tissues were up-regulated compared to the head and larval tissues;while CaN,Notum and Siah-1 in the thoracic tissues were up-regulated compared to the abdominal tissues. In sum,these genes regulation may be related to the muscle development and wings morphogenesis of the black cutworm.

Key words: Agrotis ipsilon, Wnt signaling pathway, transcriptome

ZHENG Wei-gang, JIN Ming-hui, SWAPAN Chakrabarty, XIAO Bin, XIAO Yu-tao, YUAN Hai-bin. Difference Expression Analysis in Different Tissues of Agrotis ipsilon Reveals the Possible Mechanism of Wing Development[J]. Biotechnology Bulletin, 2019, 35(10): 102-110.

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