Abstract: This work is to investigate the bioinformatics analysis and subcellular localization of human HSPA8 gene. The HSPA8 gene CDS region was amplified by RT-PCR method,and its physicochemical properties,secondary structure and tertiary structure were analyzed by bioinformatics tools and its phylogenetic tree was constructed. The recombinant eukaryotic expression vector pEGFP-C1-HSPA8 was constructed by restriction enzyme digestion and ligation,and transfected into HEK-293T cells. The subcellular localization was observed by fluorescence colocalization. The result showed that the CDS region of human HSPA8 gene was successfully cloned. The results of bioinformatics analysis showed that the human HSPA8 gene CDS region was 1 941 bp in length and the molecular formula was C₃₁₁₁H₄₉₉₈N₈₆₀O₉₉₄S₁₇. The relative molecular weight was 70.89 kD,encoding a total of 646 amino acids. The secondary structure prediction showed HSPA8 protein consisted of the α-helix（41.64%）and random coil（32.20%）dominantly. HSPA8 nucleotide homology and genetic phylogenetic tree analysis showed that humans and chimpanzees were closely related. Fluorescence colocalization results showed that HSPA8 protein was evenly distributed throughout the cell. The bioinformatics analysis and subcellular localization of HSPA8 protein laid the foundation for further exploring the intracellular function of HSPA8 gene.

Key words: HSPA8, gene cloning, bioinformatics, subcellular localization