The Interaction Law Between Small Molecular Substances and Aptamers

doi:10.13560/j.cnki.biotech.bull.1985.2019-1106

Abstract

Abstract: After nearly 30 years of development,nucleic acid aptamers have gradually become one of the hotspots in many subject areas such as biology,medicine,materials,and physics. The trace detection of small molecular substances is also of great significance in various fields,thus it is necessary to understand the action mode and law between small molecules and aptamers. In this paper,based on the classification of small molecular substances,the action modes between small molecular substances and aptamers are summarized,the existing methods of studying interactions between small molecules and nucleic acids are expounded,and the types of interaction between different target molecules and aptamers are discussed. The results indicate that the aptamer segment tends to "wrap" the target small molecule,allowing the target to "set" in the nucleic acid structure. Small molecule compounds often utilize hydrogen bonding to bind aptamers,and there are hydrophobic and electrostatic interactions between the two. The metal target molecule binds to the negatively charged moiety of the pyrimidine group of the aptamer. This paper classifies the interaction modes and binding sites between small molecules and aptamers,aiming to play a reference and guide for the development of small molecule aptamers.

Key words: small molecules, aptamer, interaction

PENG Yuan-yuan, XIAO Xing-ning, ZHU Long-jiao, TAO Xiao-qi, XU Wen-tao. The Interaction Law Between Small Molecular Substances and Aptamers[J]. Biotechnology Bulletin, 2020, 36(8): 201-209.

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