Effects of Bacteriophages DCEAV-31 and DCEIV-9 on the Algicidal Characteristics of Algicidal Bacterium Against Microcystis

doi:10.13560/j.cnki.biotech.bull.1985.2022-0009

Abstract

Abstract:

Water bloom is a global concerned environmental issue，and Microcystis is the main algae causing water bloom. The growth and decline of Microcystis is closely related to the physico-chemical and biotic factors in aquatic ecosystems，while the regulatory effects of bacteriophage infecting algicidal bacterium to Microcystis remains insufficient understanding. The aim of this study is to understand the interactions among Microcystis，algicidal bacterium and bacteriophage. In this study，the biological characteristics of Exiguobacterium bacteriophage DCEAV-31 isolated from Dianchi Lake were studied，and interactions among Microcystis - Exiguobacterium - bacteriophage were investigated. DCEAV-31 belonged to the Siphoviridae. Its burst size was 28 PFU/infected cell and the host range was narrow. DCEAV-31 was sensitive to temperature and pH，extremely sensitive to chloroform，ethanol，proteinase K，and SDS，but not sensitive to Triton X-100. The co-cultivation of Microcystis-Exiguobacterium-bacteriophage showed that phages reduced the algal lysis activity of algicidal bacterium to Microcystis，and bacteriophages also directly inhibited the proliferation of Microcystis. These results indicated that bacteriophages regulate the proliferation of Microcystis by lysis algicidal bacterium，which provides a new perspective for us to understand the growth and decline of Microcystis.

Key words: bacteriophage, Microcystis aeruginosa, algicidal bacterium, interaction, Exiguobacterium

ZHANG Jun-feng, LI Meng-ke, WU Zhi-hao, CUI Xiao-long, XIAO wei, ZHANG Shi-ying. Effects of Bacteriophages DCEAV-31 and DCEIV-9 on the Algicidal Characteristics of Algicidal Bacterium Against Microcystis[J]. Biotechnology Bulletin, 2022, 38(11): 250-257.

Figures/Tables 4

Table 1 Different assemblage setups for co-culture experi-ments

试验编号 Test No.	藻 Algae	菌 Bacterium	噬菌体 Bacteriophage
试验A	M.aeruginosa 905
试验B	M.aeruginosa 905	EA-31/EI-9
试验C	M.aeruginosa 905		DCEAV-31/DCEIV-9
试验D	M.aeruginosa 905	EA-31/EI-9	DCEAV-31/DCEIV-9

Fig. 1 TEM image（A）and one-step growth curve（B）of phage DCEAV-31

Fig. 2 Effects of pH（A）and temperature（B）on phage DCEAV-31

Fig. 3 Concentration of cells and virus particles in different experimental groups A，B：Cells concentration of M. aeruginosa 905. C，D：Cells concentration of Exiguobacterium EA-31 and EI-9. E，F：Titer of phage DCEAV-31 and DCEIV-9

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