Study on the Tolerance of Saccharomyces cerevisiae Strain to Phenolic Inhibitors

doi:10.13560/j.cnki.biotech.bull.1985.2019-1200

Abstract

Abstract: Short-term domestication strategy can be used to improve the tolerance of Saccharomyces cerevisiae to phenols produced in lignocellulose pretreatment. The inhibition of phenolic acids to the yeast strain was investigated,the physiological indexes of the domesticated strains were compared,and the membrane integrity of yeast cell was examined using the flow cytometer. The sole phenolic acid at low concentration presented insignificant inhibition on the growth and fermentation of S. cerevisiae,while significant inhibition at high concentration. Multiple phenolic acids demonstrated stronger inhibition,especially on the ethanol fermentation. Compared to the original strain,the growth and fermentation kinetic parameters of the short-term domesticated strains under mixed phenolic acid stress were significantly improved,and cell membrane remained fine integrity. Phenolic acids directly inhibited the growth of S. cerevisiae,and the short-term domestication effectively improved the tolerance of S. cerevisiae to phenolic acids,this is related to that the stress response of cell membrane remained its integrity.

Key words: cellulosic ethanol, phenolic inhibitors, Saccharomyces cerevisiae, stress response

GU Han-qi, LIU Ran, SHAO Ling-zhi, XU Yan-yan, WANG Dong-yan, ZHANG Dong-mei, LI Jie. Study on the Tolerance of Saccharomyces cerevisiae Strain to Phenolic Inhibitors[J]. Biotechnology Bulletin, 2020, 36(6): 136-142.

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