Research Progress of CRISPR /Cas9 Gene Editing Technology in Goat and Sheep

doi:10.13560/j.cnki.biotech.bull.1985.2020-0068

Abstract

Abstract: CRISPR/cas9 system is a new gene editing technology in recent years,and it can precisely edit and modify the specific DNA sequence of organism,including knockout,insertion or site-directed mutation. It is simple,efficient,precise and specific. Therefore,it has been widely used in biomedicine,gene engineering and genetic breeding research. The goat and sheep are important economic domestic animal and experimental animal. Genetic modification of sheep and goat by CRISPR/cas9 editing system can accelerate breed improvement,increase livestock production and obtain high quality agricultural and sideline products. Here we mainly summarize the overview,mechanism and the research progress of CRISPR/cas9 gene editing technology and its application in “humanized” modification of goat milk,raising meat quality,improving wool fiber quality,and the prospect,aiming at providing reference for relevant researchers.

Key words: CRISPR/cas9, gene editing, genome, genetics, goat, sheep

SONG Shao-zheng, LU Rui, ZHANG Ting, HE Zheng-yi, WU Zhao-manqiu, CHENG Yong, ZHOU Ming-ming. Research Progress of CRISPR /Cas9 Gene Editing Technology in Goat and Sheep[J]. Biotechnology Bulletin, 2020, 36(3): 62-68.

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