Total Carbohydrates and β-glucans Accumulation of Rhodosorus sp. SCSIO-45730

doi:10.13560/j.cnki.biotech.bull.1985.2020-0639

Abstract

Abstract:

Rhodosorus sp. has the potential to be used in medicine,healthcare and bioenergy due to its capacity of accumulating high carbohydrates content. In order to evaluate its industrial potential,the growth,total carbohydrates and β-glucans accumulation of this strain in 4 different culture media with different salinities were investigated. Results showed that the modified ASW medium was more beneficial for the biomass and total carbohydrates accumulation in Rhodosorus sp. SCSIO-45730 compared to other media（f/2,Conway and Erdschreiber）. The salinity adaptability of this alga was studied using ASW medium as the basic medium. Results indicated that this alga grew normally in the salinity of 18‰-58‰. The cell size gradually increased and the algal cell aggregated with the increase of salinity. The highest biomass,total carbohydrates content and productivity,and β-glucans productivity of Rhodosorus sp. SCSIO-45730 were obtained at the salinity of 38‰,which was 13.70 g/L,48.52% DW,412.58 mg/L·d and 119.70 mg/L·d,respectively,while the β-glucans content decreased with the increase of salinity. The total carbohydrates and β-glucans volumetric yield of this alga in outdoor flat-plate photobioreactor were 0.73 g/L and 0.23 g/L,respectively. In conclusion,Rhodosorus sp. SCSIO-45730 is capable of adaptation to a wide range of salinity and has high potential for total carbohydrates and β-glucans production. It is considered as an optimal feedstock for the production of bioethanol and β-glucans.

Key words: Rhodosorus, salinity, total carbohydrates, bioethanol, β-glucans

DAI Lu-mei, LI Tao, WU Hua-lian, WU Hou-bo, XIANG Wen-zhou. Total Carbohydrates and β-glucans Accumulation of Rhodosorus sp. SCSIO-45730[J]. Biotechnology Bulletin, 2021, 37(1): 205-214.

Figures/Tables 8

References 30

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	f/2 medium	Conway medium	Erdschreiber medium	ASW medium
NaNO₃/（g·L^-1）	1.50	1.50	1.50	1.50
NaH₂PO₄/（g·L^-1）	0.01	0.03	0.03	/
K₂HPO₄/（g·L^-1）	/	/	/	0.12
NaHCO₃/（g·L^-1）	0.50	/	/	0.04
FeCl₃·6H₂O/（mg·L^-1）	3.16	1.30	1.16	3.15
Na₂EDTA·2H₂O/（mg·L^-1）	4.36	45	/	4.36
MnCl₂·4H₂O/（mg·L^-1）	0.18	0.36	0.50	0.18
ZnCl₂/（mg·L^-1）	/	4.20	0.06	/
ZnSO₄·7H₂O/（mg·L^-1）	0.023	/	/	0.022
CoCl₂·6H₂O/（mg·L^-1）	0.012	4	0.024	0.01
CuSO₄·5H₂O/（mg·L^-1）	0.01	4	/	0.01
（NH₄）₆Mo₇O₂₄·4H₂O/（mg·L^-1）	/	1.80	/	/
Na₂MoO₄·2H₂O/（mg·L^-1）	0.07	/	0.048	0.006
H₃BO₃/（mg·L^-1）	/	33.4	/	/
Thiamin HCl（VB1）/（mg·L^-1）	/	0.20	0.20	/
VB₁₂/（mg·L^-1）	/	0.10	0.10	/
Salinity/‰	25	36	36	28

	f/2 medium	Conway medium	Erdschreiber medium	ASW medium
NaNO₃/（g·L^-1）	1.50	1.50	1.50	1.50
NaH₂PO₄/（g·L^-1）	0.01	0.03	0.03	/
K₂HPO₄/（g·L^-1）	/	/	/	0.12
NaHCO₃/（g·L^-1）	0.50	/	/	0.04
FeCl₃·6H₂O/（mg·L^-1）	3.16	1.30	1.16	3.15
Na₂EDTA·2H₂O/（mg·L^-1）	4.36	45	/	4.36
MnCl₂·4H₂O/（mg·L^-1）	0.18	0.36	0.50	0.18
ZnCl₂/（mg·L^-1）	/	4.20	0.06	/
ZnSO₄·7H₂O/（mg·L^-1）	0.023	/	/	0.022
CoCl₂·6H₂O/（mg·L^-1）	0.012	4	0.024	0.01
CuSO₄·5H₂O/（mg·L^-1）	0.01	4	/	0.01
（NH₄）₆Mo₇O₂₄·4H₂O/（mg·L^-1）	/	1.80	/	/
Na₂MoO₄·2H₂O/（mg·L^-1）	0.07	/	0.048	0.006
H₃BO₃/（mg·L^-1）	/	33.4	/	/
Thiamin HCl（VB1）/（mg·L^-1）	/	0.20	0.20	/
VB₁₂/（mg·L^-1）	/	0.10	0.10	/
Salinity/‰	25	36	36	28