Research Progress on Cellulase Immobilized by Magnetic Nanoparticles as Carriers

doi:10.13560/j.cnki.biotech.bull.1985.2015.08.009

Abstract

Abstract: Conversion of biomass to reducing sugar by cellulase is the foundation of the biomass fuel production. Nowadays researchers have invented the enzyme immobilization technology to avoid the disadvantages of free enzymatic hydrolization. Utilization of magnetic nanoparticles as carriers for the cellulase immobilization can increase the catalytic activities of enzyme and enhance stability of the enzyme. Moreover, replacing conventional mechanical stirring by external magnetic field can give full play to the role of carriers’ magnetic response; with the immobilization on magnetic nanoparticles, the produced cellulase can be separated easily from the mixture in the reaction systems. Researchers have proposed many excellent immobilization methods. Here the different methods of cellulase immobilization by magnetic nanoparticles are reviewed, their applications are explained in detail, and consequently the advantages and disadvantages as well as the development prospect are discussed.

Key words: bioethanol, cellulase, cellulase immobilization, magnetic nanoparticles as carrier

Xing Zhaohui, Su Yuelong, Zhang Qi, Ruan Xinyi, Lin Yan, Wang Xinze, Kong Hainan. Research Progress on Cellulase Immobilized by Magnetic Nanoparticles as Carriers[J]. Biotechnology Bulletin, 2015, 31(8): 59-65.

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