Gene Cloning and Expression Analysis of Fesod Gene from Chlorella pyrenoidosa

doi:10.13560/j.cnki.biotech.bull.1985.2015.05.027

Abstract

Abstract: The Fesod gene of Chlorella pyrenoidosa was cloned by RACE method. Total 1 215 bp Fesod sequence was obtained, containing 52 bp 5' untranslated region, 452 bp 3' untranslated region, and an open reading frame of 711 bp. The bioinformatics analysis showed that the molecular weight of FeSOD was 26.42 kD and isoelectric point was 6.98; the FeSOD protein located in the mitochondria with a probability of 73.9%; the FeSOD protein had no signal peptide; and the secondary structure prediction results showed that a-helix and random coil in FeSOD accounted for 53.39% and 39.41%, respectively. Then, the transcriptional expression of Fesod at different concentrations of salinity and salicylic acid was detected by real-time fluorescent quantitative PCR. The results showed that Fesod expression increased with the salinity varying from 15‰ to 45‰, and the expression quantity was 3.61 times under 45‰ salinity culture than that under 15‰ salinity. Moreover, salicylic acid could inhibit the mRNA accumulation of Fesod to a certain extent under 45‰ salinity, and its accumulation firstly increased and then decreased with the salicylic acid concentration from 0.1 to 2.0 mmol/L. In conclusion, the expression of Fesod was induced by salinity, while salicylic acid showed no significant effect on its expression.

Key words: Chlorella pyrenoidosa, Fe-superoxide dismutase, transcriptional expression, salinity, salicylic acid

Shen Jia, Jiang Lingzhi, Sun Xue. Gene Cloning and Expression Analysis of Fesod Gene from Chlorella pyrenoidosa[J]. Biotechnology Bulletin, 2015, 31(5): 173-178.

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