Application Effect of a Novel Plant Immune Inducer ZNC in Tobacco

doi:10.13560/j.cnki.biotech.bull.1985.2020-0673

Abstract

Abstract:

Immune inducer ZhiNengCong（ZNC）,an ethanol extract of endophyte Paecilomyces variotii,has function of promoting plant growth and enhancing disease resistance. Our study aims to evaluate the induced disease resistance and growth and development effect of ZNC on tobacco while ZNC were sprayed at the different stage of tobacco. The results showed that the 3 concentrations of ZNC showed fine control effect on viral diseases,and reduced the occurrence of rhizome diseases and later leaf spot diseases. The control effect of 500 ng/mL ZNC on tobacco virus and brown spot was the best,reaching 57.07%,indicating that this inducer had a favorable inducement effect on the main tobacco diseases. Compared with CK,the yield increased 6.00% and 5.41% respectively in 50 ng/mL and 500 ng/mL treatments,and the content of total sugar,reducing sugar,total nitrogen and K₂O also increased in 500 ng/mL,i.e.,increasing yield and improving quality occurred at certain level. More experiment demonstrated that 1 000 ng/mL ZNC treatment showed anti-disease effect,however,it presented significantly inhibitory effect on tobacco growth. This showed that ZNC had a fine effect on tobacco growth and disease resistance within a certain dose range,and further experiments are needed to determine it.

Key words: immune inducer, ZNC, tobacco, disease resistance, growth and development

GUO Mei-yan, LIU Bao-you, LI Yang, ZHANG Xiao-ying, CEHN Yu-guo, DING Xin-hua. Application Effect of a Novel Plant Immune Inducer ZNC in Tobacco[J]. Biotechnology Bulletin, 2021, 37(1): 182-188.

Figures/Tables 7

References 30

[1]	王志德, 张兴伟, 王元英, 刘艳华. 中国烟草种质资源目录(续编一)[M]. 北京: 中国农业科学技术出版社, 2018.
	Wang ZD, Zhang XW, Wang YY, Liu YH. Catalogue of tobacco germplasm resources in China[M]. Beijing: China Agricultural Science and Technology Press, 2018.
[2]	罗熹, 王家忠, 赖东辉, 等. 烟草农药残留研究进展[J]. 山东化工, 2019,48(3):27-28.
	Luo X, Wang JZ, Lai DH, et al. Research progress on tobacco pesticide residues[J]. Shandong Chemical Industry, 2019,48(3):27-28.
[3]	Qiu DW, Dong YJ, Zhang Y, et al. Plant immunity inducer development and application[J]. Molecular Plant Microbe Interactions, 2017,30(5):355-360.
[4]	Jones JG, Dangl JL. The plant immune system[J]. Nature, 2006,444(7117):323-329. doi: 10.1038/nature05286 URL pmid: 17108957
[5]	邱德文. 植物免疫诱抗剂的研究进展与应用前景[J]. 中国农业科技导报, 2014,16(1):39-45.
	Qiu DW. Progress and prospect of plant immunity inducer[J]. Journal of Agricultural Science and Technology, 2014,16(1):39-45.
[6]	刘权, 李广悦, 曾洪梅, 等. 微生物蛋白激发子PeaT1的获得及诱导水稻抗旱性的初步研究[J]. 中国农业科技导报, 2009,11(3):51-55.
	Liu Q, Li GY, Zeng HM, et al. Acquisition of microbial protein elicitor PeaT1 and preliminary research on inducing drought resistance of rice[J]. Journal of Agricultural Science and Technology, 2009,11(3):51-55.
[7]	张薇, 杨秀芬, 邱德文, 等. 激活蛋白PeaT1诱导烟草对TMV的系统抗性[J]. 植物病理学报, 2010,40(3):290-299.
	Zhang W, Yang XF, Qiu DW, et al. Activator protein PeaT1 induced systemic resistance to tobacco mosaic virus in tobacco[J]. Acta Phytopathologica Sinica, 2010,40(3):290-299.
[8]	张微. PeaT1诱导烟草系统获得抗病性及其作用机理的研究[D]. 北京:中国农业科学院, 2010.
	Zhang W. Induction of systemic acquired resistance to TMV with PeaT1 and the mechanism in tobacco[D]. Beijing:Chinese Academy of Agricultural Sciences, 2010.
[9]	Mahesh K, Liu H, Zhang YL, et al. Hrip1, a novel protein elicitor from necrotrophic fungus, Alternaria tenuissima, elicits cell death, expression of defence-related genes and systemic acquired resistance in tobacco[J]. Plant, Cell & Environment, 2012,35(12):2104-2120.
[10]	Aziz A, Benoit P, Xavier D, et al. Laminarin elicits defense responses in grapevine and induces protection against Botrytis cinerea and Plasmopara viticola[J]. Molecular Plant Microbe Interactions, 2003,16(12):1118. doi: 10.1094/MPMI.2003.16.12.1118 URL pmid: 14651345
[11]	商文静, 吴云锋, 赵小明, 等. 壳寡糖诱导烟草抗烟草花叶病毒的超微结构研究[J]. 植物病理学报, 2007,37(1):56-61.
	Shang WJ, Wu YF, Zhao XM, et al. Ultrastructure of induced resistance of tobacco to tobacco mosaic virus by chitooligosaccharides[J]. Acta Phytopathologica Sinica, 2007,37(1):56-61.
[12]	李堆淑, 胡景江. 寡聚糖激发子对杨树防御酶系活性的影响[J]. 西北林学院学报, 2012,27(2):26-29.
	Li DS, Hu JJ. Effect of the activities of defense enzymes on poplar by oligosaccharides elicitors[J]. Journal of Northwest Forestry University, 2012,27(2):26-29.
[13]	Phuntumart V, Marro P, Jean-Pierre M, et al. A novel cucumber gene associated with systemic acquired resistance[J]. Plant Science, 2006,171(5):555-564.
[14]	Radwan DM, Lu GQ, Fayez KA, et al. Protective action of salicylic acid against bean yellow mosaic virus infection in Vicia faba leaves[J]. Journal of Plant Physiology, 2008,165(8):845-857. doi: 10.1016/j.jplph.2007.07.012 URL pmid: 17920158
[15]	Lu CC, Liu HF, Jiang DP, et al. Paecilomyces variotii extracts(ZNC)enhance plant immunity and promote plant growth[J]. Plant and Soil, 2019,441(1-2):383-397.
[16]	赵鹏, 王洪凤, 刘娟, 等. 含氨基酸水溶肥料(智能聪)对马铃薯性状和产量的影响[J]. 现代农业科技, 2018(21):67-69.
	Zhao P, Wang HF, Liu J, et al. Effect of amino acid-containing water-soluble fertilizer(ZhiNengCong)on potato traits and yield[J]. XianDai NongYe Keji, 2018(21):67-69.
[17]	齐林锁. 水稻应用智能聪水溶肥料试验效果[J]. 现代化农业, 2016(3):39-40.
	Qi LS. Experimental effect of applying water-soluble fertilizer ZhiNengCong to rice[J]. Modernizing Agriculture, 2016(3):39-40.
[18]	范志金, 刘秀峰, 刘凤丽, 等. 植物抗病激活剂诱导植物抗病性的研究进展[J]. 植物保护学报, 2005,32(1):87-92.
	Fan ZJ, Liu XF, Liu FL, et al. Progress of researches on induced resistance of plant activator[J]. Journal of Plant Protection, 2005,32(1):87-92.
[19]	余清, 刘勇, 莫笑晗, 等. 氨基寡糖素在烟草上的应用[J]. 中国生物防治, 2002,18(3):128-131.
	Yu Q, Liu Y, Mo XH, et al. Applying amino-oligosaccharin on tobacco for controlling tobacco virus disease[J]. Chinese Journal of Biological Control, 2002,18(3):128-131.
[20]	苏小记, 贾丽娜. 2. 0%氨基寡糖素水剂防治烟草病毒病药效试验[J]. 陕西农业科学, 2005(3):55-56.
	Su XJ, Jia LN. Control effect of 2. 0% amino-oligosaccharin AS on tobacco virus[J]. Shanxi Journal of Agricultural Sciences, 2005(3):55-56.
[21]	罗刚, 高华军, 韦忠, 等. 氨基寡糖素和钾营养调节剂对烟草普通花叶病毒病的防治效果[J]. 作物研究, 2018,32(2):140-143.
	Luo G, Gao HJ, Wei Z, et al. The control efficiency of amino-oligosaccharins and potassium nutrition regulator on tobacco mosaic virus disease[J]. Crop Research, 2018,32(2):140-143.
[22]	沈晗, 杨凯, 任伟, 等. 影响上部烟叶感官质量的主要化学成分分析[J]. 中国烟草学报, 2019,5(6):18-26.
	Shen H, Yang K, Ren W, et al. Analysis of essential chemical components affecting sensory quality of upper tobacco leaves[J]. Acta Tabacaria Sinica, 2019,25(6):18-26.
[23]	Peterson RL, Wagg C, Pautler M. Associations between microfungal endophytes and roots:Do structural features indicate function?[J] Botany, 2008,86:445-456.
[24]	Della Mónica IF, Saparrat MC, Godeas AM, et al. The co-existence between DSE and AMF symbionts affects plant P pools through P mineralization and solubilization processes[J]. Fungal Ecology, 2015,17:10-17.
[25]	Nguyen TT, Paul NC, Lee HB. Characterization of Paecilomyces variotii and Talaromyces amestolkiae in Korea based on the morphological characteristics and multigene phylogenetic analyses[J]. Mycobiology, 2016,44:248-259. URL pmid: 28154482
[26]	Nakajima M, Itoi K, Takamatsu Y, et al. Cornexistin:A new fungal metabolite with herbicidal activity[J]. Journal of Antibiotics, 1991,44:1065-1072.
[27]	Song X, Zhang LH, Peng AT, et al. First report of Paecilomyces variotii isolated from Citrus Psyllid(Diaphorina citri), the vector of Huanglongbing of Citrus, in China[J]. Plant Disease, 2016,100:2526.
[28]	Huang WK, Cui JK, Liu SM, et al. Testing various biocontrol agents against the root-knot nematode(Meloidogyne incognita)in cucumber plants identifies a combination of Syncephalastrum racemosum and Paecilomyces lilacinus as being most effective[J]. Boil Control, 2016,92:31-37.
[29]	Moreno-Gavíra A, Diánez F, Sánchez-Montesinos B, et al. Paecilomyces variotii as a plant-growth promoter in horticulture[J]. Agronomy, 2020,10:597.
[30]	Peng C, Zhang A, Wang Q, et al. Ultrahigh-activity immune inducer from endophytic fungi induces tobacco resistance to virus by SA pathway and RNA silencing[J]. BMC Plant Biology, 2020. DOI: 10. 1186/s12870-020-02386-4. URL pmid: 33451304

处理	株高/cm	茎围/cm	有效叶片数/片	最大叶面积/cm²
CK	9.28±0.25b	1.76±0.06 a	6.28±0.17 a	145.62±2.14 c
50 ng/mL	9.85±0.30 a	1.76±0.07 a	6.28±0.16 a	155.83±2.72 a
500 ng/mL	9.25±0.56 b	1.77±0.05 a	6.33±0.10 a	148.25±3.64 b
1000 ng/mL	8.95±0.20 c	1.73±0.07 a	6.23±0.17 a	141.56±3.65 d

处理	株高/cm	茎围/cm	有效叶片数/片	最大叶面积/cm²
CK	9.28±0.25b	1.76±0.06 a	6.28±0.17 a	145.62±2.14 c
50 ng/mL	9.85±0.30 a	1.76±0.07 a	6.28±0.16 a	155.83±2.72 a
500 ng/mL	9.25±0.56 b	1.77±0.05 a	6.33±0.10 a	148.25±3.64 b
1000 ng/mL	8.95±0.20 c	1.73±0.07 a	6.23±0.17 a	141.56±3.65 d

处理	发病率/%	平均病指	防效/%
CK	12.70±1.56	3.89±0.39
50 ng/mL	11.25±1.25	2.36±0.14	39.33±8.85 c
500 ng/mL	7.50±1.44	1.67±0.39	57.07±6.06 a
1000 ng/mL	7.50±1.44	1.94±0.58	50.13±18.23 b

处理	发病率/%	平均病指	防效/%
CK	12.70±1.56	3.89±0.39
50 ng/mL	11.25±1.25	2.36±0.14	39.33±8.85 c
500 ng/mL	7.50±1.44	1.67±0.39	57.07±6.06 a
1000 ng/mL	7.50±1.44	1.94±0.58	50.13±18.23 b

处理	株高/cm	茎围/cm	节间距/cm	有效叶片数/片	最大叶面积/cm²
CK	34.18±1.81 b	8.80±0.28 a	3.89±0.34 a	10.60±0.71 a	753.05±20.49 c
50 ng/mL	38.26±1.56 a	8.68±0.44 a	3.98±0.29 a	10.38±0.30 a	786.99±14.38 a
500 ng/mL	37.54±1.08 a	8.58±0.56 a	3.65±0.23 a	10.68±0.82 a	774.24±24.61 b
1000 ng/mL	31.93±2.01 c	8.83±0.70 a	3.28±0.21 a	10.30±0.47 a	723.20±23.06 d