Study on Oxygen Release and Photosynthetic Rate of Immobilized Chlorella

doi:10.13560/j.cnki.biotech.bull.1985.2020-0860

Abstract

Abstract:

To further solve the problem of insufficient dissolved oxygen in aquaculture and lay the foundation for the application of biological oxygenation technology,the oxygen release and photosynthetic rate of immobilized chlorella were studied. Because of its high biomass and biological activity,immobilization had been widely used in research. The oxygen production and photosynthetic rate of immobilized chlorella were studied by immobilization technique. The results showed that the immobilized chlorella biomass was higher than the free chlorella’s,composite immobilized material（sodium carboxymethyl cellulose +sodium alginate + diatomite）was conducive to the release of oxygen on chlorella and its top release between 3-4 d,the optimal immobilized chlorella volume was 150 mL providing oxygen for 1 L water,and net photosynthetic rate of immobilized chlorella was obviously higher than that of free chlorella. The immobilization increases the biomass and biological activity of chlorella,and has better biological oxygenation characteristics,it is a fine biological oxygenation material.

Key words: immobilization, chlorella, dissolved oxygen

LU Shang-de, LIU Jing-jing, FENG Yi-ping, ZHAO Peng, XU Yang-cang. Study on Oxygen Release and Photosynthetic Rate of Immobilized Chlorella[J]. Biotechnology Bulletin, 2021, 37(3): 92-98.

Figures/Tables 9

References 26

[1]	沈英, 张华, 赵云, 等. 吸附式微藻固定化培养技术研究进展[J]. 海洋科学, 2013,37(11):109-115.
	Shen Y, Zhang H, Zhao Y, et al. Advances in attached microalgae culture technology[J]. Marine Science, 2013,37(11):109-115.
[2]	俞志明, 陈楠生. 国内外赤潮的发展趋势与研究热点[J]. 海洋与湖沼, 2019,50(3):474-486.
	Yu ZM, Chen NS. Emerging trends in red tide and major research progrssses[J]. Oceanologia et Limnologia Sinica, 2019,50(3):474-486.
[3]	周国民. 我国农业大数据应用进展综述[J]. 农业大数据学报, 2019,1(1):16-23.
	Zhou GM. Progress in the application of big data in agriculture in China[J]. Journal of Agricultural Big Data, 2019,1(1):16-23.
[4]	刘翀, 岳冬冬, 刘兴国. 中国水产养殖业“走出去”的现状、存在问题与对策[J]. 渔业信息与战略, 2018,33(4):235-239.
	Liu C, Yue DD, Liu XG. Reflection on “Going Out” for Chinese aquaculture[J]. Fishery Infornation & Strategy, 2018,33(4):235-239.
[5]	续钊. 浅析水产养殖水体污染及微生物修复[J]. 生物化工, 2018,4(6):134-141.
	Xu Z. Analysis of aquaculture water pollution and microbial remediation[J]. Biological Chemical Engineering, 2018,4(6):134-141.
[6]	李明. 浙江省水产养殖机械化现状及机械装备需求[J]. 农业工程, 2016,6(1):1-4.
	Li M. Current situation of mechanization production and requirement of mechanized equipment about aquaculture industry in zhejiang province[J]. Agricultural Engineering, 2016,6(1):1-4.
[7]	王春德, 齐翠红, 李朝霞. 过氧化钙的净水增氧效果评估[J]. 生命科学研究, 2012,16(4):345-349.
	Wang CD, Qi CH, Li ZX. Evaluation of calcium peroxide as a new water purifying and oxygen releasing agent in aquaculture[J]. Life Science Reaesrch, 2012,16(4):345-349.
[8]	李永函, 雷衍之, 张桂兰. 鱼类越冬中生物增氧技术的研究[J]. 淡水渔业, 1984(4):25-29.
	Li YH, Lei YZ, Zhang GL. Study on biological oxygenation techno-logy in overwintering fish[J]. Freshwater Fishery, 1984(4):25-29.
[9]	于霞, 柴立元, 甘雪萍. 细胞固定化技术及其在废水处理中的应用研究[J]. 工业水处理, 2002,21(10):11-14, 42.
	Yu X, Chai LY, Gan XP. Present state and prospect of cell immobilization technique and its application to wastewater treatment[J]. Industrial Water Treatment, 2001,21(10):11-14, 42.
[10]	Kube M, Spedding B, Gao L, et al. Nutrient removal by alginate-immobilized chlorella vulgaris:response to different wastewater matrices[J]. Journal of Chemical Technology and Biotechnology, 2020,95(6):1790-1799.
[11]	Jimenez P, Sanchez C, Romerao O, et al. Growth and nutrient removal in free and immobilized planktonic green isolated from manure[J]. Enzyme and Microbial Technology, 2004,34(5):392-398. doi: 10.1016/j.enzmictec.2003.07.010 URL
[12]	Zhang LJ, Ying GG, Chen F. Development and application of whole-sediment toxiciity test using immobilized freshwater microalgae pseudokirchneriella subcapitata[J]. Environmental Toxicology and Chemistry, 2012,31(2):377-386. doi: 10.1002/etc.734 URL
[13]	杨海波, 于媛, 张欣华, 李英敏. 小球藻固定化培养的初步研究[J]. 水产科学, 2001,20(5):4-7.
	Yang HB, Yu Y, Zhang XH, Li YM. Study on immobilization vulture of marine microagla Chlorella vulgaris[J]. Fisheries Science, 2001,20(5):4-7.
[14]	郎筱宇, 刘志媛, 徐梦, 等. 葡萄糖对小球藻(Chloralla sp. HN08)光合作用和生长的影响[J]. 微生物学报, 2017,57(4):550-559. doi: 10.13343/j.cnki.wsxb.20160329 URL
	Lang XY, Liu ZY, Xu M, et al. Effects of glucose on photosynjournal and growth of Chloralla sp. HN08 cells[J]. Acta Microbiologica Sinica, 2017,57(4):550-559.
[15]	王帅, 王玥, 梁英, 等. PVC 塑料浸出液对3种海洋微藻光合作用及生长的影响[J]. 生态毒理学报, 2020,15(2):113-129.
	Wang S, Wang Y, Liang Y, et al. Effects of PVC plastic leachate on the photosynjournal and growth of three marine microalgae[J]. Asian Journal of Ecotoxicology, 2020,15(2):113-129.
[16]	汪洋, 孔维宝, 张馨允, 等. 海藻酸钙凝胶包埋法制备固定化小球藻[J]. 生物学通报, 2015,50(9):50-53.
	Wang Y, Kong WB, Zhang XY, et al. Immobilized chlorella was made by calcium alginate gel embedding[J]. Bulletin of Biology, 2015,50(9):50-53.
[17]	张瑞朋, 付连舜, 佟斌, 等. 大豆叶片光合作用与光强及二氧化碳的关系[J]. 吉林农业科学, 2015,40(3):8-13.
	Zhang RP, Fu LS, Tong B, et al. The relationship of soybean leaf photosynjournal with light intersity and carbon dioxide[J]. Journal of Jilin Agricultural Sciences, 2015,40(3):8-13.
[18]	刘群. 浅谈生态养殖技术在水产养殖中的应用[J]. 农业技术与装备, 2020,363(3):157-158.
	Liu Q. On the application of ecological culture technology in aquaculture[J]. Agricultural Technology & Equipment, 2020,363(3):157-158.
[19]	王传印, 包东娥, 李新艳, 等. 红富士苹果叶片净光合速率与生理生态因子的关系[J]. 落叶果树, 2018,50(1):8-10.
	Wang CY, Bao DE, Li XY, et al. Relationship between net photosynthetic rate and physiological and ecological factors in leaves of Fuji Apple[J]. Deciduous Fruits, 2018,50(1):8-10.
[20]	张雨晴, 于海业, 刘爽, 等. 玉米叶片净光合速率快速检测方法研究[J]. 农机化研究, 2019,41(4):182-185.
	Zhang YQ, Yu HY, Liu S, et al. Study on rapid detection method of net photosynthetic rate of maize leaves[J]. Journal of Agricultural Mechanization Research, 2019,41(4):182-185.
[21]	林海, 周刚, 周军, 等. 池塘养殖增氧方式效果比较[J]. 水产养殖, 2010,31(5):41-44.
	Lin Hi, Zhou G, Zhou J, et al. Contrastive study on the effects of different methods of aeration[J]. Journal of Aquaculture, 2010,31(5):41-44.
[22]	李潇轩, 杨志强, 尹陆乐, 等. 2种增氧模式对锦鲤生长的效果分析[J]. 水产养殖, 2019,40(6):14-16.
	Li XX, Yang ZQ, Yi LL, et al. Analysis of the effects of two aerobic modes on the growth of Koi carp[J]. Aquaculture, 2019,40(6):14-16.
[23]	沈正洲. 苏南地区水产增氧机械化现状分析与对策思考[J]. 现代农机, 2013(3):20-21.
	Shen ZZ. Analysis and countermeasure thinking of the present situation of the aquatic product oxygenation mechanization in Southern Jiangsu province[J]. The Modern Agricultural Machinery, 2013(3):20-21.
[24]	李海玲, 陈丽华, 肖朝虎, 哈斯其美格. 微生物固定化载体材料的研究进展[J]. 现代化工, 2020,40(8):58-66.
	Li HL, Chen LH, Xiao CH, et al. Research progress in microorganisms immobilized carrier materials[J]. Modern Chemical Industry, 2020,40(8):58-66.
[25]	胡骏, 林炜铁, 罗剑飞. 琼脂包埋微藻用于处理氮磷废水的研究[J]. 广州化工, 2020,48(10):74-78.
	Hu J, Lin WT, Luo JF. Study on the treatment of Nitrogen and phosphorus wastewater by AGAR embedded microalgae[J]. Guangdong Chemical Industy, 2020,48(10):74-78.
[26]	吴义诚, 张建发, 刘征, 等. 生物炭-海藻酸钠联合固定化小球藻去除水中的氨氮[J]. 环境工程学报, 2019,13(12):2863-2869.
	Wu YC, Zhang JF, Liu Z, et al. Removal of ammonia nitrogen by biochar-alginate-jointly immobilized Chlorella Vulgaris[J]. Chinese Journal of Environmental Engineering, 2019,13(12):2863-2869.