Abstract: A fungus isolated and purified from soil was identified as Aspergillus fumigatus L-3. Using strain L-3 as immobilized carrier,on which Bacillus Licheniformis was immobilized to form an immobilized system. The degradation of Congo red dye wastewater by the composite mycelial cake,mycelia,fermentation mixture,and crude laccase enzyme solution,as well as the toxicity test of the dye wastewater were conducted. The results showed that the degradation effect on the dye wastewater by mycelial pellets was the most significant,the degradation rate reached 99.96%;and the degradation rate by mycelia cake was 91% with only 20 s,while the degradation effect by the fermentation mixture and the crude laccase solution was not so significant. The detoxification rate from dye wastewater by this system was obvious,especially the detoxification rate by mycelial pellet reached 78%. It can be seen that the immobilized system has not only high degradation ability but also a high detoxification rate.

Key words: Aspergillus fumigatus, immobilization, Congo red, toxicity, mycelial pellet