Abstract:

Genome-wide identification and expression pattern analysis of Solanum tuberosum bHLH transcription factor were conducted in order to provide reference for studying its biological function. Based on the S. tuberosum genome database and Pfam database,HMMER was used to identify the S. tuberosum bHLH family members through the whole genome. After the redundant sequences were excluded,ExPASy and MEME software were used to analyze the basic physicochemical properties and conservative element analysis of the candidate sequences. Further MEGA-X software was adapted for cluster analysis. MG2C and TBtools were employed to depict chromosome location and expression pattern respectively. As result,a total of 108 bHLH transcription factors were retrieved from the S. tuberosum genome database,the number of amino acids ranged from 62 to 694,the molecular weight was 7 527.78-75 939.94 Da,and the theoretical isoelectric point was 4.55-10.40. These bHLH transcription factors were distributed on 12 chromosomes of S. tuberosum,and all had typical HLH domain,which could be divided into 16 subgroups. Gene expression pattern analysis showed that S. tuberosum bHLH family members had tissue expression specificity and most of them responded to salt,mannitol,IAA,ABA,GA₃ and heat stress. The whole genome of S. tuberosum contains 108 highly conserved members of the bHLH gene family,whose expression is tissue specific and responds to the induction of abiotic stress.

Key words: Solanum tuberosum, bHLH transcription factor, genomic identification, stress, expression analysis