Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (8): 203-212.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1410

Previous Articles     Next Articles

Cloning,Expression and Bioinformatics Analysis of the CDS Region of Chicken CEBPA Gene

DU Zhen-wei(), ZHU Shuai-peng, MA Xiang-fei, LI Dong-hua(), SUN Gui-rong   

  1. College of Animal Science and Technology,Henan Agricultural University,Zhengzhou 540046
  • Received:2020-11-19 Online:2021-08-26 Published:2021-09-10
  • Contact: LI Dong-hua E-mail:x13592547117@126.com;lidonghua6656@126.com

Abstract:

This work is to investigate the amino acid sequence characteristics of CCAAT/Enhancer Binding Protein Alpha(CEBPA)and the mRNA expression profile of its coding gene in different tissues of Gushi chicken. The CDS sequence of CEBPA with a total length of 975 bp was cloned by PCR,and it encoded 325 amino acids. The bioinformatics analyses showed that there was no transmembrane structure and signal peptide in CEBPA,and the protein structure was mainly composed of irregular crimp and α-spiral,and most of them were distributed in the nucleus. The construction of protein interaction network indicated that CEBPA might interact with TRBs family and Runx transcription factors family. Amino acid sequence comparison found that chicken and quail were closely related and had 13 different promoters and a 1 640 bp CpG island on its -2 000 bp sequence of the 5' regulatory region. qRT-PCR results showed that the expression of CEBPA gene in the breast muscle and leg muscle was significantly lower than that in other tissues of 12-week Guishi chickens(P<0.05). The expression of CEBPA in the breast muscle of 22-week chicken was significantly lower than that in 6-week and 55-week ones(P < 0.05). The expression of CEBPA in the breast muscle of Ross chicken was always significantly higher than that in the breast muscle of Gushi chicken(P<0.05). The above results provide a theoretical basis of further exploring the biological function of chicken CEBPA gene in the lipid deposition process of chicken breast muscle.

Key words: Gushi chicken, CEBPA gene, bioinformatics analysis, breast muscle, expression analysis