Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (8): 213-220.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0055

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Comprehensive Transcriptome Analysis of Intestinal Epithelial Cells of Cyprinus carpio Exposed to Lipopolysaccharide

CHEN Jian-jun1(), ZHAO Yi-di1, CAO Xiang-lin2()   

  1. 1. College of Life Science,Henan Normal University,Xinxiang 453007
    2. College of Fisheries,Henan Normal University,Xinxiang 453007
  • Received:2021-01-13 Online:2021-08-26 Published:2021-09-10
  • Contact: CAO Xiang-lin E-mail:cjjjianjun@163.com;hsdcxl@163.com

Abstract:

This work aims to explore the effect of lipopolysaccharide(LPS)on the genes and functions of intestinal epithelial cells in Cyprinus carpio. The intestinal epithelial cells of C. carpio were taken as the research object,normal treatment was as the control group,and lipopolysaccharide treatment was as the experimental group. Transcriptome sequencing was performed on the two groups of the intestinal epithelial cells of C. carpio treated for 24 h. A total 44.77G of high-quality data from transcriptome sequencing results were obtained,of which,nearly 81.83% data could be aligned to the C. carpio genome. Analyzed using the DESeq software and compared with the control group,590 differentially expressed genes(DEG)were induced by LPS treatment. Among them,303 were up-regulated and 287 were down-regulated. Gene Ontology(GO)functional enrichment analysis showed that DEG accounted for the highest proportion in cellular processes,single organism processes,metabolic processes,cells,cell part,organelle,binding and catalytic activity functional subclasses. The Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis revealed that DEG was significantly enriched in cell cycle,autophagy and apoptosis pathways. The 10 randomly selected differential genes were verified by quantitative real-time PCR,and the results showed that the transcriptome data were reliable. In this study,transcriptome sequencing technology was used to comprehensively and rapidly obtain all transcriptome information during LPS exposure to C. carpio intestinal epithelial cells,and to systematically prove that LPS blocked the cell cycle of C. carpio intestinal epithelial cells,induced autophagy and apoptosis,thus providing an important theoretical basis for the molecular mechanism of LPS regulation in C. carpio intestinal epithelial cells.

Key words: lipopolysaccharide, Cyprinus carpio, intestinal epithelial cells, transcriptome sequencing, quantitative real-time PCR