Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (8): 203-212.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1410
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DU Zhen-wei(), ZHU Shuai-peng, MA Xiang-fei, LI Dong-hua(), SUN Gui-rong
Received:
2020-11-19
Online:
2021-08-26
Published:
2021-09-10
Contact:
LI Dong-hua
E-mail:x13592547117@126.com;lidonghua6656@126.com
DU Zhen-wei, ZHU Shuai-peng, MA Xiang-fei, LI Dong-hua, SUN Gui-rong. Cloning,Expression and Bioinformatics Analysis of the CDS Region of Chicken CEBPA Gene[J]. Biotechnology Bulletin, 2021, 37(8): 203-212.
目的基因Gene name | 引物序列 Primer sequence(5'-3') | 用途 Purpose |
---|---|---|
CEBPA | F:ATGGAGCAAGCCAACTTCTACGAGG R:GGCGCAGCTGCCCATGGCCTTCAC | 基因克隆Genetic cloning |
CEBPA | F:TTCTACGAGGTCGATTCCCG R:AGCCTCTCTGTAGCCGTAG | 基因表达分析Gene expression analysis |
β-actin | F:CAGCCAGCCATGGATGATGA R:ACCAACCATCACACCCTGAT | 内参基因Endogeneity |
Table 1 Gene primer sequences
目的基因Gene name | 引物序列 Primer sequence(5'-3') | 用途 Purpose |
---|---|---|
CEBPA | F:ATGGAGCAAGCCAACTTCTACGAGG R:GGCGCAGCTGCCCATGGCCTTCAC | 基因克隆Genetic cloning |
CEBPA | F:TTCTACGAGGTCGATTCCCG R:AGCCTCTCTGTAGCCGTAG | 基因表达分析Gene expression analysis |
β-actin | F:CAGCCAGCCATGGATGATGA R:ACCAACCATCACACCCTGAT | 内参基因Endogeneity |
Fig.1 Cloning results of chicken CEBPA CDS region A:PCR amplification results of chicken CEBPA conserved sequences;B:sequencing results of positive colonies compared with CDS sequences of CEBPA on NCBI
Fig.2 Prediction of CEBPA protein structure and function A:Transmembrane structural domain of chicken CEBPA protein. B:Signal peptide of chicken CEBPA protein. C:Secondary structure of chicken CEBPA protein. D:Tertiary structure of chicken CEBPA protein. E:Chicken CEBPA protein interaction network
Fig.3 Conserved amino acid sequence analysis of CEBPA A:Comparison of the homology of the amino acid sequences of CEBPA in different species. B:Phylogenetic tree analysis of CEBPA in different species
Fig.4 Schematic diagram of the predicted structure of CEBPA promoter and CpG island A:Schematic diagram of CEBPA promoter prediction. B:Methylation CpG islands predicted by EMBOSS software. C:Methylation CpG islands predicted by MethPrimer software
Fig.5 Spatial and temporal expression profiles of CEBPA in Gushi chickens Different lowercase letters indicate significant differences between different tissues for comparison(P<0.05),the same below
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