Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (10): 63-71.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0136

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Study on the Reduction Characteristics of Cr(VI)by Two Species of Microorganisms

HUANG Yu-xi(), CHENG Shun-li, HE Ling-ling, XIAO Jin-bin, REN Qiu-he, PENG Zi-han, ZHOU Zhen, FANG Yu-mei()   

  1. Henan High-tech Industrial Co. Ltd.,Zhengzhou 450002
  • Received:2021-02-02 Online:2021-10-26 Published:2021-11-12
  • Contact: FANG Yu-mei E-mail:huangyuxi92@163.com;fymnumber1@126.com

Abstract:

This work aims to study the reduction ability and characteristics of two species of microorganisms to Cr(VI). The abilities of two species of microorganisms to reduce Cr(VI)and the influence of pH and electron donor on its reduction of Cr(VI)were explored by continuous culturing in lab condition,combining characterization methods the detoxification mechanism of Stenotrophomonas sp. was studied The results showed that the tolerance of Stenotrophomonas sp. and Bacillus mucilaginosus to Cr(VI)was 400 and 200 mg/L. Under neutral conditions,the removal rate of 45.5 and 18.5 mg/L Cr(VI)within 72 h was 100%. Under weakly alkaline conditions,the activities of both microorganisms were strong. At pH 8,removal rates of 50 mg/L Cr(VI)by Stenotrophomonas sp. and B. mucilaginosus within 72 h were 98.1% and 49.2% respectively. Sodium acetate and sodium lactate promoted the removal rate of 50 mg/L Cr(VI)by Stenotrophomonas sp. to be 100% within 60 h. Sodium lactate and glucose increased the removal rate of 50 mg/L Cr(VI)by B. mucilaginosus from 49.2% to 61.9% and 73.2% within 72 h,respectively. The analysis of the reduced products showed that Stenotrophomonas sp. adsorbed Cr(VI)on the surface of the body and efficiently reduced Cr(VI)to Cr(III). Stenotrophomonas sp. and B. mucilaginosus can be used as high quality strain for remediation of heavy metal Cr(VI)pollution.

Key words: heavy metal pollution, microorganism, Cr(VI), adsorption, reduction