Cloning and Expression Pattern Analysis of Jasmonic Acid Receptor Gene McCOI1a in Mentha canadensis L.

doi:10.13560/j.cnki.biotech.bull.1985.2023-0789

Abstract

Abstract:

【Objective】The jasmonic acid（JA）receptor COI1 plays a vital role in regulating plant growth and development, and stress response in plants. Cloning and analysis of the protein characterization, and analyzing expressions of McCOI1a from Mentha canadensis L. may provide the gene resources for its molecular breeding. 【Method】The McCOI1a gene was cloned from the leaves of M. canadensis based on transcriptome data, and then the characteristics, subcellular localization, and expression profiles of McCOI1a were analyzed through bioinformatics analysis, transient expression in the leaves of tobacco, and real-time quantitative PCR. 【Result】The full-length of McCOI1a gene was 1 842 bp and it encoded 613 amino acids. McCOI1a protein possessed the conserved F-box and LRR domains and that was highly homologous with Salvia miltiorrhiza SmCOI1. Subcellular localization results showed that McCOI1a protein was located in the nucleus. The qRT-PCR results showed that McCOI1a was broadly expressed in different tissues, with the highest expression in the root; McCOI1a expression gradually increased during leaf development. In addition, the expressions of McCOI1a in the leaves were up-regulated to varying degrees under MeJA, drought, NaCl, AlCl₃, CdCl₂, and CuCl₂ treatments, and the up-regulation was the most obvious under AlCl₃treatment, with the highest expression reaching 1 206-fold. And McCOI1a expression in the root was firstly down-regulated and then up-regulated under CuCl₂ treatment, while the expression of McCOI1a showed varying degrees of down-regulation under other treatments. 【Conclusion】McCOI1a responds to MeJA and abiotic stress, and may play an important role in regulating the growth and development, and stress response in M. canadensis.

Key words: Mentha canadensis L., McCOI1a gene, protein characteristics, stress response, expression analysis

TANG Wei-lin, KANG Qin, WANG Xia, SHEN Ming-yang, SUN Xin-jiang, WANG Ke, HOU Kai, WU Wei, XU Dong-bei. Cloning and Expression Pattern Analysis of Jasmonic Acid Receptor Gene McCOI1a in Mentha canadensis L.[J]. Biotechnology Bulletin, 2024, 40(1): 270-280.

Figures/Tables 8

Fig. 1 McCOI1a gene and its protein structure analysis in M. canadensis A: The diagram of McCOI1a gene and the conserved domain of McCOI1a protein. a: The full-length of McCOI1a; b: the full-length sequence of protein and the positions of conserved domain F-box, TIR1, and AMN1; c: the scale bar of the length of amino acid; bp: base pair. B: Secondary structure of McCOI1a protein. Blue: α-helix; Green: beta-turn; Red: extended-strand; Purple: random coil. C: Tertiary structure of McCOI1a protein. Baby blue: α-helix. Purple: beta-turn; Green: the residue around the ligand; Red: ligand; The dotted yellow line: hydrogen bond

Fig. 2 Amino acid sequence comparison of McCOI1a and its homologous proteins Black box: F-box domain; red underline: leucine-rich repeat sequence（LRR）

Fig. 3 Analysis of conserved motifs of McCOI1a and its homologous proteins

Fig. 4 Phylogenetic analysis of COI1 proteins from M. canadensis and other species

Fig. 5 Subcellular localization results of McCOI1a

Fig. 6 Expression analysis of McCOI1a gene in different tissues and phyllotaxis of M. canadensis A: Expressions of McCOI1a in the roots, stems, young leaf, old leaf, flower, bud, apical shoot of M. canadensis. B: Relative expressions of McCOI1a in the leaves at different positions. Leaf 1-leaf 8: The first to eighth leaf morphologically from top to bottom. The data are of ±SE of three biological replicates

Fig. 7 Expression analysis of McCOI1a in the leaves of M. canadensis under MeJA and abiotic stress treatments The data ±SE（n=3）of three biological replicates. Duncan test was used in ANOVA, the lowercase letters indicate significant differences at the 0.05 level. The same below

Fig. 8 Expression analysis of McCOI1a in the roots of M. canadensis under MeJA and abiotic stress treatments

References 39

[1]	陈智坤, 梁呈元, 任冰如, 等. 薄荷属植物挥发性成分及药理作用研究进展[J]. 天然产物研究与开发, 2013, 25(6): 856-861, 865.
	Chen ZK, Liang CY, Ren BR, et al. Advances in the studies of chemical constituents and pharmacological activities of volatile components from Mentha L[J]. Nat Prod Res Dev, 2013, 25(6): 856-861, 865.
[2]	Yu X, Liang CY, Chen J, et al. The effects of salinity stress on morphological characteristics, mineral nutrient accumulation and essential oil yield and composition in Mentha canadensis L[J]. Sci Hortic, 2015, 197: 579-583. doi: 10.1016/j.scienta.2015.10.023 URL
[3]	Azimychetabi Z, Sabokdast Nodehi M, Karami Moghadam T, et al. Cadmium stress alters the essential oil composition and the expression of genes involved in their synthesis in peppermint(Mentha piperita L.)[J]. Ind Crops Prod, 2021, 168: 113602. doi: 10.1016/j.indcrop.2021.113602 URL
[4]	Singh R, Luxmi S, Charak A, et al. Effects of intermittent drought on the essential oil yield, contents, and nutrient status of Mentha longifolia(L.) huds[J]. J Essent Oil Bear Plants, 2022, 25(3): 626-638. doi: 10.1080/0972060X.2022.2091957 URL
[5]	Yu XX, Zhang WJ, Zhang Y, et al. The roles of methyl jasmonate to stress in plants[J]. Funct Plant Biol, 2019, 46(3): 197-212. doi: 10.1071/FP18106 pmid: 32172764
[6]	Yan JB, Zhang C, Gu M, et al. The Arabidopsis CORONATINE INSENSITIVE1 protein is a jasmonate receptor[J]. Plant Cell, 2009, 21(8): 2220-2236. doi: 10.1105/tpc.109.065730 URL
[7]	Wasternack C, Song SS. Jasmonates: biosynthesis, metabolism, and signaling by proteins activating and repressing transcription[J]. J Exp Bot, 2017, 68(6): 1303-1321. doi: 10.1093/jxb/erw443 pmid: 27940470
[8]	Xie DX, Feys BF, James S, et al. COI1: an Arabidopsis gene required for jasmonate-regulated defense and fertility[J]. Science, 1998, 280(5366): 1091-1094. doi: 10.1126/science.280.5366.1091 pmid: 9582125
[9]	Ye M, Luo SM, Xie JF, et al. Silencing COI1 in rice increases susceptibility to chewing insects and impairs inducible defense[J]. PLoS One, 2012, 7(4): e36214. doi: 10.1371/journal.pone.0036214 URL
[10]	Lee SH, Sakuraba Y, Lee T, et al. Mutation of Oryza sativa CORONATINE INSENSITIVE 1b(OsCOI1b)delays leaf senescence[J]. J Integr Plant Biol, 2015, 57(6): 562-576. doi: 10.1111/jipb.v57.6 URL
[11]	Bai JF, Wang YK, Wang P, et al. Genome-wide identification and analysis of the COI gene family in wheat(Triticum aestivum L.)[J]. BMC Genomics, 2018, 19(1): 754. doi: 10.1186/s12864-018-5116-9
[12]	Qi XL, Guo SW, Wang D, et al. ZmCOI2a and ZmCOI2b redundantly regulate anther dehiscence and gametophytic male fertility in maize[J]. Plant J, 2022, 110(3): 849-862. doi: 10.1111/tpj.v110.3 URL
[13]	Sun TT, Meng YT, Cen GL, et al. Genome-wide identification and expression analysis of the coronatine-insensitive 1(COI1)gene family in response to biotic and abiotic stresses in Saccharum[J]. BMC Genomics, 2022, 23(1): 38. doi: 10.1186/s12864-021-08255-0
[14]	Chen QF, Dai LY, Xiao S, et al. The COI1 and DFR genes are essential for regulation of jasmonate-induced anthocyanin accumulation in Arabidopsis[J]. J Integr Plant Biol, 2007, 49(9): 1370-1377. doi: 10.1111/jipb.2007.49.issue-9 URL
[15]	Shen Q, Lu X, Yan TX, et al. The jasmonate-responsive AaMYC2 transcription factor positively regulates artemisinin biosynthesis in Artemisia annua[J]. New Phytol, 2016, 210(4): 1269-1281. doi: 10.1111/nph.2016.210.issue-4 URL
[16]	Zhou YY, Sun W, Chen JF, et al. SmMYC2a and SmMYC2b played similar but irreplaceable roles in regulating the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza[J]. Sci Rep, 2016, 6: 22852. doi: 10.1038/srep22852
[17]	Zhang HT, Hedhili S, Montiel G, et al. The basic helix-loop-helix transcription factor CrMYC2 controls the jasmonate-responsive expression of the ORCA genes that regulate alkaloid biosynthesis in Catharanthus roseus[J]. Plant J, 2011, 67(1): 61-71. doi: 10.1111/tpj.2011.67.issue-1 URL
[18]	Zhang HB, Bokowiec MT, Rushton PJ, et al. Tobacco transcription factors NtMYC2a and NtMYC2b form nuclear complexes with the NtJAZ1 repressor and regulate multiple jasmonate-inducible steps in nicotine biosynthesis[J]. Mol Plant, 2012, 5(1): 73-84. doi: 10.1093/mp/ssr056 URL
[19]	Xu DB, Ma YN, Qin TF, et al. Transcriptome-wide identification and characterization of the JAZ gene family in Mentha canadensis L[J]. Int J Mol Sci, 2021, 22(16): 8859. doi: 10.3390/ijms22168859 URL
[20]	Ma YN, Xu DB, Li L, et al. Jasmonate promotes artemisinin biosynthesis by activating the TCP14-ORA complex in Artemisia annua[J]. Sci Adv, 2018, 4(11): eaas9357. doi: 10.1126/sciadv.aas9357 URL
[21]	Ruan JJ, Zhou YX, Zhou ML, et al. Jasmonic acid signaling pathway in plants[J]. Int J Mol Sci, 2019, 20(10): 2479. doi: 10.3390/ijms20102479 URL
[22]	Chini A, Boter M, Solano R. Plant oxylipins: COI1/JAZs/MYC2 as the core jasmonic acid-signalling module[J]. FEBS J, 2009, 276(17): 4682-4692. doi: 10.1111/j.1742-4658.2009.07194.x pmid: 19663905
[23]	Trang Nguyen H, Thi Mai To H, Lebrun M, et al. Jasmonates-the master regulator of rice development, adaptation and defense[J]. Plants, 2019, 8(9): 339. doi: 10.3390/plants8090339 URL
[24]	Chen J, Yang HT, Ma S, et al. HbCOI1 perceives jasmonate to trigger signal transduction in Hevea brasiliensis[J]. Tree Physiol, 2021, 41(3): 460-471. doi: 10.1093/treephys/tpaa124 URL
[25]	Hu S, Yu KM, Yan JB, et al. Jasmonate perception: ligand-receptor interaction, regulation, and evolution[J]. Mol Plant, 2023, 16(1): 23-42. doi: 10.1016/j.molp.2022.08.011 URL
[26]	付鸿博, 向成刚, 陶宏征, 等. 灯盏花COI1基因的克隆及表达分析[J]. 分子植物育种, 2023. http://kns.cnki.net/kcms/detail/46.1068.s.20220707.1441.006.html.
	Fu HB, Xiang CG, Tao HZ, et al. Cloning and expression analysis of COI1 gene in Erigeron breviscapus[J]. Molecular Plant Breeding, 2023. http://kns.cnki.net/kcms/detail/46.1068.s.20220707.1441.006.html.
[27]	Lee HY, Seo JS, Cho JH, et al. Oryza sativa COI homologues restore jasmonate signal transduction in Arabidopsis coi1-1 mutants[J]. PLoS One, 2013, 8(1): e52802. doi: 10.1371/journal.pone.0052802 URL
[28]	吴晨雨, 何俊娜, 钟雄辉, 等. 唐菖蒲茉莉素受体基因GhCOI1的克隆与表达分析[J]. 园艺学报, 2015, 42(2): 289-300.
	Wu CY, He JN, Zhong XH, et al. Cloning and expression analysis of coronatine insensitive gene encoding a jasmonate receptor from Gladiolus hybridus[J]. Acta Hortic Sin, 2015, 42(2): 289-300.
[29]	Shan XY, Wang JX, Chua L, et al. The role of Arabidopsis Rubisco activase in jasmonate-induced leaf senescence[J]. Plant Physiol, 2011, 155(2): 751-764. doi: 10.1104/pp.110.166595 URL
[30]	Chen YN, Feng PP, Tang BY, et al. The AP2/ERF transcription factor SlERF.F5 functions in leaf senescence in tomato[J]. Plant Cell Rep, 2022, 41(5): 1181-1195. doi: 10.1007/s00299-022-02846-1 pmid: 35238951
[31]	Liao YC, Wei JH, Xu YH, et al. Cloning, expression and characterization of COI1 gene(AsCOI1)from Aquilaria sinensis(Lour.) Gilg[J]. Acta Pharm Sin B, 2015, 5(5): 473-481. doi: 10.1016/j.apsb.2015.05.009 URL
[32]	Liu R, Wang JB, Xiao M, et al. AaCOI1, encoding a CORONATINE INSENSITIVE 1-like protein of Artemisia annua L., is involved in development, defense, and anthocyanin synthesis[J]. Genes, 2020, 11(2): 221. doi: 10.3390/genes11020221 URL
[33]	Yastreb TO, Kolupaev YE, Shkliarevskyi MA, et al. Involvement of jasmonate signaling components in salt stress-induced stomatal closure in Arabidopsis thaliana[J]. Cytol Genet, 2020, 54(4): 318-323. doi: 10.3103/S009545272004012X
[34]	Gupta A, Bhardwaj M, Tran LS P. JASMONATE ZIM-DOMAIN family proteins: important nodes in jasmonic acid-abscisic acid crosstalk for regulating plant response to drought[J]. Curr Protein Pept Sci, 2021, 22(11): 759-766. doi: 10.2174/1389203722666211018114443 URL
[35]	Chen YJ, Chen Y, Shi ZJ, et al. Biosynthesis and signal transduction of ABA, JA, and BRs in response to drought stress of Kentucky bluegrass[J]. Int J Mol Sci, 2019, 20(6): 1289. doi: 10.3390/ijms20061289 URL
[36]	Valenzuela CE, Acevedo-Acevedo O, Miranda GS, et al. Salt stress response triggers activation of the jasmonate signaling pathway leading to inhibition of cell elongation in Arabidopsis primary root[J]. J Exp Bot, 2016, 67(14): 4209-4220. doi: 10.1093/jxb/erw202 pmid: 27217545
[37]	Yang ZB, He CM, Ma YQ, et al. Jasmonic acid enhances Al-induced root growth inhibition[J]. Plant Physiol, 2017, 173(2): 1420-1433. doi: 10.1104/pp.16.01756 URL
[38]	Zhang CH, Huang RQ, Zhan NH, et al. Methyl jasmonate and selenium synergistically mitigative cadmium toxicity in hot pepper(Capsicum annuum L.) plants by improving antioxidase activities and reducing Cd accumulation[J]. Environ Sci Pollut Res Int, 2023, 30(34): 82458-82469. doi: 10.1007/s11356-023-28273-7
[39]	Hanaka A, Wójcik M, Dresler S, et al. Does methyl jasmonate modify the oxidative stress response in Phaseolus coccineus treated with Cu?[J]. Ecotoxicol Environ Saf, 2016, 124: 480-488. doi: 10.1016/j.ecoenv.2015.11.024 URL