Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (4): 264-270.doi: 10.13560/j.cnki.biotech.bull.1985.2023-1040

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Biological Characteristics, Domestication and Cultivation of Wild Gymnopilus subpurpuratus

RAO Yong-bin1(), ZHANG Jun-li2()   

  1. 1. Sanming Agricultural and Rural Bureau,Sanming 365000
    2. Vegetable Research Institute of Xizang Autonomous Academy of Agriculture and Animal Husbandry Sciences, Lhasa 850000
  • Received:2023-11-07 Online:2024-04-26 Published:2024-04-30
  • Contact: ZHANG Jun-li E-mail:26493933@qq.com;594686187@qq.com

Abstract:

Objective】This work is to realize the efficient development and utilization of Xizang’s wild fungi resources and promote the development and growth of edible and medicinal fungi industry.【Method】A wild fungus collected fromYigong township, Bomi county, Linzhi city, Xizang autonomous region was isolated and purified, and the strain was identified according to morphological characteristics and its sequence.【Result】Combined with morphological characteristics and ITS phylogenetic tree results, the strain was identified as Gymnopilus subpurpuratus. The results show that the best carbon source for mycelium growth is glucose, the best nitrogen source is yeast powder, the optimal pH is 6.0, and the optimal temperature is 30℃. The results of domestication show that the filling-bag time in the culture medium with sawdust as the main cultivation material is 39 d, the formation time of primordium is 18 d, the harvest time is 8 dafter primordium was out, and the fresh weight of single fungus is 45.99 g.【Conclusion】To sum up, the artificial domesticated fruiting body of G. subpurpuratus is successfully obtained by using sawdust as the main cultivation material, which increases the new species of artificial domestication of medicinal fungi.

Key words: Gymnopilus subpurpuratus, identification, biological characteristics, domestication and cultivation