Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (4): 228-241.doi: 10.13560/j.cnki.biotech.bull.1985.2023-1172

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Efficacy and Its Mechanism of Bacterial Strain HX0037 on the Control of Anthracnose Disease of Trichosanthes kirilowii Maxim

XU Wei-fang1,2(), LI He-yu1, ZHANG Hui1, HE Zi-ang1, GAO Wen-heng1, XIE Zi-yang1, WANG Chuan-wen3, YIN Deng-ke1()   

  1. 1. School of Pharmacy, Anhui University of Chinese Medicine, Anhui Province Key Laboratory of Research & Development of Chinese Medicine, Hefei 230012
    2. Joint Research Center for Chinese Herbal Medicine of Anhui of IHM, Anhui University of Chinese Medicine, Hefei 230012
    3. County Qianshan Chuan-Wen Trichosanthes Seeds Technology Co. Ltd, Anqing 246300
  • Received:2023-12-13 Online:2024-04-26 Published:2024-04-30
  • Contact: YIN Deng-ke E-mail:xuweifang@ahtcm.edu.cn;yindengke@ahtcm.edu.cn

Abstract:

Objective】The biocontrol ability of bacterium HX0037 against anthracnose disease of Trichosanthes kirilowii Maxim and its biocontrol mechanism were studied. 【Method】The antagonistic activities of HX0037 against four pathogens of fruit anthracnose disease were determined in petri dishes experiments. The biocontrol efficiencies of HX0037 against Trichosanthes leaf anthracnose and Trichosanthes fruit anthracnose were evaluated on detached leaves and fruits in vitro. In addition, HX0037 was further identified based on the analyses on morphological features, physiological and biochemical characteristics and molecular biological identification results of the whole genome, and the secondary metabolites of biocontrol bacteria HX0037 were also predicted. The lipopeptide compounds produced by strain HX0037 were extracted by acid precipitation and analyzed by high-performance liquid chromatography tandem mass spectrometry(LC-MS). Finally, the possible biocontrol mechanism was explored through two experiments, observing the effects of lipopeptides produced by strain HX0037 on the hyphal growth and morphology of Colletotrichum gloeosporioides, and the abilities of strain HX0037 to produce enzyme and siderophore. 【Result】Strain HX0037 showed varied antimicrobial activity against four pathogens of fruit anthracnose disease, especially the best against C. gloeosporioides, a pathogen of Trichosanthes anthracnose disease, and it obviously changed the cell membrane permeability of the pathogenic fungi. Compared with plant tissues inoculated with the pathogen alone, inoculation with strain HX0037 significantly reduced the incidence of leaf and fruit anthracnose diseases of T. kirilowii Maxim, and the biocontrol efficacy was 61.50% and 52.51%, respectively. Strain HX0037 was further identified as Bacillus amyloliquefaciens, and its genome contained 12 secondary metabolite gene clusters, and it decomposed the protein, cellulose and starch, and also produced siderophore. The hyphae of C. gloeosporioides were distorted, intumescent, broken and more transparent after treatment with the antifungal lipopeptide produced by strain HX0037. Surfactin and fengycin were detected from the lipopeptide extracts of strain HX0037 by LC-MS. 【Conclusion】The B. amyloliquefaciens HX0037 has strong antagonistic activity on C. gloeosporioides, and is expected to be further developed as a biological agent for the biocontrol of Trichosanthes anthracnose diseases.

Key words: Bacillus amyloliquefaciens, biological control, Trichosanthes anthracnose disease, antagonism, whole genome, species identification, lipopeptide