Advances in the Molecular Mechanisms of Plant Tissue Culture and Regeneration Regulated by Totipotency-related Transcription Factors

doi:10.13560/j.cnki.biotech.bull.1985.2023-1164

Abstract

Abstract:

Plant cells demonstrate the potential to develop into completely new organisms under suitable culture conditions, a phenomenon known as plant cell totipotency. Tissue culture techniques based on this totipotency have been extensively applied in the fields of asexual reproduction and genetic improvement through genetic transformation in plants. Abiotic stress, plant hormones and transcription factors synergistically regulate plant regeneration in vitro. Among these, transcription factors that play a pivotal role in plant regeneration are referred to as totipotency-related transcription factors. Recent advancements have shed light on the molecular mechanisms by which these totipotency-related transcription factors regulate plant in vitro regeneration. Numerous transcription factors have been identified and preliminarily applied in studies aimed at enhancing the efficiency of plant genetic transformation. According to the function of plant cell totipotent transcription factors in plant regeneration, this paper summarizes the signal transduction mechanism in plant regeneration in vitro in recent years, summarizes the role of plant cell totipotent transcription factors in improving the efficiency of plant genetic transformation, and looks forward to its application prospect, so as to provide scientific basis for establishing an effective asexual propagation system.

Key words: totipotency-related transcription factors, tissue culture, in vitro regeneration, regulatory mechanisms

WANG Di ZHANG Xiao-yu SONG Yu-xin ZHENG Dong-ran TIAN Jing LI Yu-hua WANG Yu WU Hao. Advances in the Molecular Mechanisms of Plant Tissue Culture and Regeneration Regulated by Totipotency-related Transcription Factors[J]. Biotechnology Bulletin, 2024, 40(6): 23-33.

Figures/Tables 3

Fig. 1 Expression forms of plant cell totipotency in plant tissue culture and regeneration

Table 1 Tertiary structure prediction diagram of major transcription factor domains

转录因子名称Name of transcription factor	结构域示意图Schematic diagram of domain	三级结构预测图Tertiary structure prediction diagram
Homeobox
AP2/ERF
LOB
LEC

Fig. 2 Regulation mechanism of related transcription factors in plant tissue culture regeneration A: Regulation mechanism of related transcription factors during callus formation; B: regulation mechanism of related transcription factors in shoot regeneration; C: regulation mechanism of related transcription factors during root meristem formation; D: regulation mechanism of related transcription factors in somatic embryogenesis

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