Abstract: Sorghum（Sorghum bicolor L. Moench）is the fifth most widely planted cereal crop in the world. It is the raw material in food,fodder and processing industry. As well,it has been identified as a potential biofuel crop. Despite the important application value,the genetic improvement of sorghum via genetic engineering technology is very difficult,because there is no highly efficient sorghum tissue culture system. According to recent studies,we reviewed the main factors having great influences on sorghum tissue culture,such as genotypes,explants,media,plant hormones,etc. Importantly,we focused on the strategies which may promote the regeneration of sorghum. At the end,two directions for solving the difficulties in sorghum regeneration via tissue culture are given as：first,the genetic and epigenetical mechanisms of plant regeneration should be studied deeply;second,the culture conditions should be further optimized based on the metabolism characteristic of every genotype. These ideas will be references for the establishment of highly efficient sorghum tissue culture system.

Key words: plant tissue culture, genotype, explant, media, plant hormone