Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (6): 23-33.doi: 10.13560/j.cnki.biotech.bull.1985.2023-1164

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Advances in the Molecular Mechanisms of Plant Tissue Culture and Regeneration Regulated by Totipotency-related Transcription Factors

WANG Di ZHANG Xiao-yu SONG Yu-xin ZHENG Dong-ran TIAN Jing LI Yu-hua WANG Yu WU Hao1,2()   

  1. 1. Key Laboratory of Vegetation Restoration and Reconstruction in Northeast Saline-alkali Land, Ministry of Education, Harbin 150000
    2. College of Life Science, Northeast Forestry University, Harbin 150000
  • Received:2023-12-10 Online:2024-06-26 Published:2024-06-24

Abstract:

Plant cells demonstrate the potential to develop into completely new organisms under suitable culture conditions, a phenomenon known as plant cell totipotency. Tissue culture techniques based on this totipotency have been extensively applied in the fields of asexual reproduction and genetic improvement through genetic transformation in plants. Abiotic stress, plant hormones and transcription factors synergistically regulate plant regeneration in vitro. Among these, transcription factors that play a pivotal role in plant regeneration are referred to as totipotency-related transcription factors. Recent advancements have shed light on the molecular mechanisms by which these totipotency-related transcription factors regulate plant in vitro regeneration. Numerous transcription factors have been identified and preliminarily applied in studies aimed at enhancing the efficiency of plant genetic transformation. According to the function of plant cell totipotent transcription factors in plant regeneration, this paper summarizes the signal transduction mechanism in plant regeneration in vitro in recent years, summarizes the role of plant cell totipotent transcription factors in improving the efficiency of plant genetic transformation, and looks forward to its application prospect, so as to provide scientific basis for establishing an effective asexual propagation system.

Key words: totipotency-related transcription factors, tissue culture, in vitro regeneration, regulatory mechanisms