Effects of Different Treatments on the Nutrients, Fiber Structure, and Glucosinolate Contents in Rapeseed Straw

doi:10.13560/j.cnki.biotech.bull.1985.2024-0077

Abstract

Abstract:

【Objective】 Physical（extrusion）and microbial（Aspergillus niger and Bacillus subtilis）treatments were applied to analyze the changes in the nutrient content, fiber structure and glucosinolates of the rapeseed straw after fermentation, aiming to improve the feeding value of rape straw as roughage for ruminants.【Method】 The experiment included 6 treatments: Rape straw group（M group, that is, control group）, control plus Aspergillus niger group（MA group）, control plus Bacillus subtilis group（MB group）, control puffed group（PM group）, control puffed plus A. niger group（PMA group）, and control puffed plus B. subtilis group（PMB group）.【Result】 Compared with the M group（2.60%）, the crude protein content significantly increased 3.16%（MA group）, 3.24%（MB group）, 3.31%（PM group）, 4.02%（PMA group）, 3.73%（PMB group）and the NDF content significantly reduced in other groups, 81.6%（MA group）, 80.3%（MB group）, 80.4%（PM group）, 77.3%（PMA group）and 78.3%（PMB group）（P<0.05）. The crystallinity（27.0% vs 25.3%）and specific surface area（1.22 m²/g vs 1.19 m²/g）in MA group was higher that than in M group（P<0.05）, the hydrogen bonding force was weakened, and there was no significant difference in the degree of polymerization in MA compared with that in the M group（P>0.05）. Compared with the M group（0.49 μmol/g）, the glucosinolate content of the other groups was significantly lower（P<0.05）. The difference in the glucosinolate contents in PMA（0.24 μmol/g）and PMBs（0.22 μmol/g）groups was not significant（P<0.05）.【Conclusion】 The use of microbial and physical treatments of rape straw can reduce the NDF level by breaking fiber structure and reducing the content of glucosinolates, thus improving its feeding value in ruminants.

Key words: rapeseed straw, extrusion, Aspergillus niger, Bacillus subtilis, fiber structure, glucosinolate

YANG Dai-yi, FAN Yang, TU Yan, XU Zhi-yu, XUE Ying-hao, SUN Yuan-feng, WANG Jin, HAO Xiao-yan, MA Tao. Effects of Different Treatments on the Nutrients, Fiber Structure, and Glucosinolate Contents in Rapeseed Straw[J]. Biotechnology Bulletin, 2024, 40(6): 172-179.

Figures/Tables 4

References 41

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项目 Item	组别 Groups						SEM	P
项目 Item	对照组 M	对照加黑曲霉组MA	对照加枯草芽孢杆菌组MB	对照膨化组PM	对照膨化加黑曲霉组PMA	对照膨化加枯草芽孢杆菌组PMB	SEM	P
干物质DM	70.5^a	67.8^b	67.3^b	70.3^a	67.2^b	64.2^b	0.40	<0.01
粗蛋白质CP	2.60^d	3.16^c	3.24^bc	3.31^bc	4.02^a	3.73^ab	0.12	<0.01
中性洗涤纤维NDF	83.4^a	81.6^b	80.3^b	80.4^b	77.3^c	78.3^c	0.52	<0.01
酸性洗涤纤维ADF	66.8^a	65.2^ab	64.1^bc	63.2^c	60.1^d	61.1^d	0.40	<0.01

项目 Item	组别 Groups						SEM	P
项目 Item	对照组 M	对照加黑曲霉组MA	对照加枯草芽孢杆菌组MB	对照膨化组PM	对照膨化加黑曲霉组PMA	对照膨化加枯草芽孢杆菌组PMB	SEM	P
干物质DM	70.5^a	67.8^b	67.3^b	70.3^a	67.2^b	64.2^b	0.40	<0.01
粗蛋白质CP	2.60^d	3.16^c	3.24^bc	3.31^bc	4.02^a	3.73^ab	0.12	<0.01
中性洗涤纤维NDF	83.4^a	81.6^b	80.3^b	80.4^b	77.3^c	78.3^c	0.52	<0.01
酸性洗涤纤维ADF	66.8^a	65.2^ab	64.1^bc	63.2^c	60.1^d	61.1^d	0.40	<0.01

项目 Item	组别 Group						SEM	P
项目 Item	对照组 M	对照加黑曲霉组MA	对照加枯草芽孢杆菌组MB	对照膨化组PM	对照膨化加黑曲霉组PMA	对照膨化加枯草芽孢杆菌组PMB	SEM	P
聚合度Degree of polymerization	467.6^a	435.1^ab	414.3^b	458.7^a	397.7^bc	366.9^c	8.04	<0.01
结晶度 Crystallinity	25.3^d	27.0^b	26.5^c	26.3^c	28.6^a	27.8^b	0.27	<0.01
比表面积 Specific surface area	1.19^d	1.22^c	1.47^b	1.46^b	1.46^b	1.72^a	0.04	<0.01

项目 Item	组别 Group						SEM	P
项目 Item	对照组 M	对照加黑曲霉组MA	对照加枯草芽孢杆菌组MB	对照膨化组PM	对照膨化加黑曲霉组PMA	对照膨化加枯草芽孢杆菌组PMB	SEM	P
聚合度Degree of polymerization	467.6^a	435.1^ab	414.3^b	458.7^a	397.7^bc	366.9^c	8.04	<0.01
结晶度 Crystallinity	25.3^d	27.0^b	26.5^c	26.3^c	28.6^a	27.8^b	0.27	<0.01
比表面积 Specific surface area	1.19^d	1.22^c	1.47^b	1.46^b	1.46^b	1.72^a	0.04	<0.01