Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (7): 299-306.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0095
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QIAO Ye1,2(), ZHANG Nan2, YANG Jian-hua2, ZHANG Cui-ying1, ZHU Lei-lei2()
Received:
2024-01-24
Online:
2024-07-26
Published:
2024-07-30
Contact:
ZHU Lei-lei
E-mail:qiaoye@tib.cas.cn;zhu_ll@tib.cas.cn
QIAO Ye, ZHANG Nan, YANG Jian-hua, ZHANG Cui-ying, ZHU Lei-lei. Identification and Enzymatic Characterization of a Sugar Phosphatase[J]. Biotechnology Bulletin, 2024, 40(7): 299-306.
Fig. 2 SDS-PAGE analysis of expression(A)and purification(B)of TsPase (A)1: The crude supernatant of pET28a(+); 2: the crude supernatant of TsPase; 3: protein marker.(B)1: The purified enzyme of TsPase; 2: protein marker
Fig. 4 Enzymatic properties of TsPase A: Catalytic activity curve of TsPase with different temperature. B: pH Curve of TsPase. C: Effect of different metal ions on the activity of TsPase. D: The catalytic activity of TsPase with different concentration of Mg2+
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