Evaluation of Drought Resistance of Three Chlorella Strains

doi:10.13560/j.cnki.biotech.bull.1985.2024-0101

Abstract

Abstract:

【Objective】In order to study the physiological response of desert Chlorella to drought stress, we isolated and cultured Chlorella with strong drought resistance, laying a foundation for the development and production of desert microalgae resources.【Method】Two strains of Chlorella vulgaris isolated from Taklimakan Desert and Gurbantunggut Desert in Xinjiang and one strain of C. vulgaris purchased from Chinese Academy of Sciences Freshwater Algae Seed Bank were used as materials, and Bold's Basal Medium（BBM）as the culture medium. The growth rate and physiological and biochemical indicators of three strains of C. vulgaris under drought stress were measured by polyethylene glycol（PEG6000）to simulate different degrees of drought stress, and the drought resistance capacity of three strains of C. vulgaris was evaluated by principal component analysis, membership function, comprehensive drought resistance measurement value and other methods.【Result】 Under drought stress, the cell density（OD₆₈₀）and biomass（dry weight）decreased, chlorophyll a and chlorophyll b content decreased, while the malondialdehyde（MDA）content and superoxide dismutase（SOD）activity increased. However, soluble sugar, protein, proline（Pro）content, and catalase（CAT）activity varied with the degree and time of stress. The principal component analysis table showed that the sugars, Pro, SOD, CAT, and chlorophyll b played important roles in the drought resistance process of C. vulgaris. According to the analysis of drought resistance coefficient, the soluble sugars, proteins, Pro, SOD, and CAT of desert Chlorella had stronger effects than ordinary ones during severe drought.【Conclusion】The comprehensive evaluation shows that the drought resistance of the three strains of Chlorella is in the following order: Desert Chlorella TLD 6B > Desert Chlorella GTD 8A1 > Common Chlorella FACHB-2338, and desert Chlorella has advantages in growth rate, sugar content, and protein content compared to common Chlorella.

Key words: desert Chlorella, drought stress, physiological index, membership function analysis, principal component analysis, drought resistance evaluation

HAN Kai, ZHOU Yong-shun, ZHANG Kai-yue, WANG Lu, GAO Jian-feng, CHEN Fu-long. Evaluation of Drought Resistance of Three Chlorella Strains[J]. Biotechnology Bulletin, 2024, 40(8): 244-254.

Figures/Tables 11

References 45

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PEG6000浓度 PEG6000 concentration/%	小球藻生物量体积产率 Biomass volume yield of C. vulgaris /（g·L^-1·d^-1）
PEG6000浓度 PEG6000 concentration/%	6B	8A1	FACHB-2338
0	0.91	0.87	0.82
5	0.82	0.78	0.73
15	0.55	0.51	0.35
25	0.22	0.16	0.08

PEG6000浓度 PEG6000 concentration/%	小球藻生物量体积产率 Biomass volume yield of C. vulgaris /（g·L^-1·d^-1）
PEG6000浓度 PEG6000 concentration/%	6B	8A1	FACHB-2338
0	0.91	0.87	0.82
5	0.82	0.78	0.73
15	0.55	0.51	0.35
25	0.22	0.16	0.08

小球藻种类 Chlorella species	OD₆₈₀	干重 Dry weight	可溶性糖 Soluble sugar	可溶性蛋白质 Soluble protein	Pro	MDA	CAT	SOD	叶绿素a Chlorophyll a	叶绿素b Chlorophyll b
6B	0.269	0.412	2.812	1.434	1.569	3.034	1.440	2.384	0.583	0.565
8A1	0.197	0.361	2.383	1.465	1.394	3.365	1.474	1.891	0.555	0.592
FACHB-2338	0.169	0.307	1.880	1.286	1.280	3.592	1.332	1.742	0.530	0.549
平均值Mean	0.212	0.36	2.358	1.359	1.414	3.330	1.415	2.005	0.558	0.569

小球藻种类 Chlorella species	OD₆₈₀	干重 Dry weight	可溶性糖 Soluble sugar	可溶性蛋白质 Soluble protein	Pro	MDA	CAT	SOD	叶绿素a Chlorophyll a	叶绿素b Chlorophyll b
6B	0.269	0.412	2.812	1.434	1.569	3.034	1.440	2.384	0.583	0.565
8A1	0.197	0.361	2.383	1.465	1.394	3.365	1.474	1.891	0.555	0.592
FACHB-2338	0.169	0.307	1.880	1.286	1.280	3.592	1.332	1.742	0.530	0.549
平均值Mean	0.212	0.36	2.358	1.359	1.414	3.330	1.415	2.005	0.558	0.569

指标 Index	PC1	PC2
OD₆₈₀	0.94	-0.34
干重Dry weight	0.997	-0.082
可溶性糖 Soluble sugar	0.999	-0.052
可溶性蛋白质 Soluble protein	0.832	0.555
脯氨酸Pro	0.976	-0.218
丙二醛MDA	-0.979	0.204
过氧化氢酶CAT	0.792	0.611
超氧化物歧化酶SOD	0.922	-0.388
叶绿素a Chlorophyll a	0.991	-0.13
叶绿素b Chlorophyll b	0.457	0.889
特征值 Eigenvalue	8.147	1.853
贡献率 Contribution rate/%	81.473	18.527
累计贡献率 Accumulated contribution rate/%	81.473	100