Abstract:

【Objective】 Genetic diversity analysis and evaluation of introduced potato varieties is a necessary method for screening good parental materials. 【Method】SSR fluorescent marker capillary electrophoresis was used to analyze the genetic diversity of 51 introduced potato varieties and construct molecular ID cards. 【Result】The 41 pairs of primers amplified a total of 327 alleles, and 311 alleles were differentiated at the same position, and the percentage of polymorphism was 95.11%. The primer polymorphism information index（PIC）ranged from 0 to 0.89, with an average of 0.71. Shannon's（I）ranged from 0 to 0.54, with an average of 0.32. The genetic similarity coefficient（GS）of the introduced potato varieties was 0.32. In the genetic similarity coefficient GS of 0.76, the test materials were categorized into six major groups: Group I having 14 varieties, group II having 30 varieties, group III having three varieties, group V having two varieties, and group IV and VI having one variety each. Cluster analysis revealed that four varieties, Vilas, Dingshu 4, Kexin 33 and Dongnong 312 could be used as potato breeding materials. 【Conclusion】Three pairs of core primers, STM0037, SSR0387 and SSR01054, were selected to construct fingerprints and molecular IDs of 51 potato varieties.

Key words: potato, SSR, capillary electrophoresis, genetic diversity, molecular identity card