Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (9): 42-50.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0509
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ZHANG Xiao-mei1(), ZHOU Nan-ling1, ZHANG Sai-hang2, WANG Chao1, SHEN Yu-long1, GUAN Jun-mei1, MA Ling1()
Received:
2024-05-29
Online:
2024-09-26
Published:
2024-10-12
Contact:
MA Ling
E-mail:1452091644@qq.com;may_ynnu@ynnu.edu.cn
ZHANG Xiao-mei, ZHOU Nan-ling, ZHANG Sai-hang, WANG Chao, SHEN Yu-long, GUAN Jun-mei, MA Ling. Cloning and Expression Analysis of StDREBs Gene in Solanum tuberosum L.[J]. Biotechnology Bulletin, 2024, 40(9): 42-50.
试剂和仪器Reagent and instrument | 用途Fuctinon | 来源Source |
---|---|---|
pRI101-AN-35S-EGFP 载体 | 构建载体 | 实验室提供 |
内切酶 EcoR I | 构建载体 | 南京诺唯赞生物科技股份有限公司 |
内切酶 Kpn I | 构建载体 | 南京诺唯赞生物科技股份有限公司 |
同源重组酶 | 构建载体 | 南京诺唯赞生物科技股份有限公司 |
PCR 高保真酶(PrimeSTAR® GXL DNA Polymerase) | 构建载体 | 宝日医生物技术(北京)有限公司 |
琼脂糖凝胶 DNA 回收试剂盒(TIANgel Purification Kit) | 构建载体 | 天根生化科技(北京)有限公司 |
大肠杆菌 DH5α | 构建载体 | 上海唯地生物技术有限公司 |
农杆菌 GV3101(pSoup-p19) | 构建载体 | 上海唯地生物技术有限公司 |
核定位染料 DAPI 溶液 | 亚细胞定位 | 北京索莱宝科技有限公司 |
TRIzol® reagent | 总RNA提取 | 维百奥(北京)生物科技有限公司 |
氯仿(三氯甲烷) | 总RNA提取 | 实验室提供 |
异丙醇 | 总RNA提取 | 天津市致远化学试剂有限公司 |
无水乙醇 | 总RNA提取 | 天津市致远化学试剂有限公司 |
cDNA 合成试剂盒 | 反转录合成 | 北京全式金生物技术股份有限公司 |
实时荧光定量试剂盒 | 定量分析 | 北京全式金生物技术股份有限公司 |
BIORADPCR 仪 | 构建载体 | 实验室提供 |
化学发光检测仪 | 构建载体 | 实验室提供 |
Zeiss 倒置共聚焦显微镜 | 亚细胞定位 | 实验室提供 |
Applied Biosystems 实时荧光定量PCR 仪 | 定量分析 | 实验室提供 |
Table 1 Reagents and instruments information
试剂和仪器Reagent and instrument | 用途Fuctinon | 来源Source |
---|---|---|
pRI101-AN-35S-EGFP 载体 | 构建载体 | 实验室提供 |
内切酶 EcoR I | 构建载体 | 南京诺唯赞生物科技股份有限公司 |
内切酶 Kpn I | 构建载体 | 南京诺唯赞生物科技股份有限公司 |
同源重组酶 | 构建载体 | 南京诺唯赞生物科技股份有限公司 |
PCR 高保真酶(PrimeSTAR® GXL DNA Polymerase) | 构建载体 | 宝日医生物技术(北京)有限公司 |
琼脂糖凝胶 DNA 回收试剂盒(TIANgel Purification Kit) | 构建载体 | 天根生化科技(北京)有限公司 |
大肠杆菌 DH5α | 构建载体 | 上海唯地生物技术有限公司 |
农杆菌 GV3101(pSoup-p19) | 构建载体 | 上海唯地生物技术有限公司 |
核定位染料 DAPI 溶液 | 亚细胞定位 | 北京索莱宝科技有限公司 |
TRIzol® reagent | 总RNA提取 | 维百奥(北京)生物科技有限公司 |
氯仿(三氯甲烷) | 总RNA提取 | 实验室提供 |
异丙醇 | 总RNA提取 | 天津市致远化学试剂有限公司 |
无水乙醇 | 总RNA提取 | 天津市致远化学试剂有限公司 |
cDNA 合成试剂盒 | 反转录合成 | 北京全式金生物技术股份有限公司 |
实时荧光定量试剂盒 | 定量分析 | 北京全式金生物技术股份有限公司 |
BIORADPCR 仪 | 构建载体 | 实验室提供 |
化学发光检测仪 | 构建载体 | 实验室提供 |
Zeiss 倒置共聚焦显微镜 | 亚细胞定位 | 实验室提供 |
Applied Biosystems 实时荧光定量PCR 仪 | 定量分析 | 实验室提供 |
引物Primer | 序列Sequence(5'-3') |
---|---|
720-CDS-F | ATGATGAAGTTATTCTTAGC |
720-CDS-R | TTATATAGAATAATTCCATA |
730-CDS-F | ATGTTTCCAAGCTATTATTC |
730-CDS-R | TTAGATTGAATAATTCCATA |
740-CDS-F | ATGAATATCTTTGAAACCTA |
740-CDS-R | TTAGATAGAATAATTCCATA |
750-CDS-F | ATGGATATCTTTCGAAGCTA |
750-CDS-R | TTAGATAGAATAGCTCCATA |
pRI101-720-F | agagttgttgattcagaattcATGATGAAGTTATTCTTAGCTTCAAATAAC |
pRI101-720-R | ctgtacaagcccgggggtaccTATAGAATAATTCCATAAAGGCATATAAGC |
pRI101-730-F | agagttgttgattcagaattcATGTTTCCAAGCTATTATTCGGAGC |
pRI101-730-R | ctgtacaagcccgggggtaccTTAGATTGAATAATTCCATAAAGACATGTAA |
pRI101-740-F | agagttgttgattcagaattcATGAATATCTTTGAAACCTATTATTCAGACC |
pRI101-740-R | ctgtacaagcccgggggtaccTTAGATAGAATAATTCCATAAAGTCATGTAAGC |
pRI101-750-F | agagttgttgattcagaattcATGGATATCTTTCGAAGCTATTATTCG |
pRI101-750-R | ctgtacaagcccgggggtaccTTAGATAGAATAGCTCCATAAAGGCATAT |
720qPCR-F | CCAACAGTAGAAATGGCAGCTAGAG |
720qPCR-R | CTTCCGACTGATCTGATGATGATGG |
730qPCR-F | GAACTTTGCTGACTCTGTTTGGAG |
730qPCR-R | CCTCATTCATAGAGAGGGCCTTTTC |
740qPCR-F | TAAAGATATTCAAAAGGCGGCCGC |
740qPCR-R | CTCTGGCGCTTCTTCGTTCATAAA |
750qPCR-F | GCTATAGCATTAAGAGGCCGTTCT |
750qPCR-R | CATCCGGAGTACTAGATTGATCTCC |
Actin-F | GGGATGGAGAAGTTTGGTGGTGG |
Actin-R | CTTCGACCAAGGGATGGTGTAGC |
Table 2 Primer sequence
引物Primer | 序列Sequence(5'-3') |
---|---|
720-CDS-F | ATGATGAAGTTATTCTTAGC |
720-CDS-R | TTATATAGAATAATTCCATA |
730-CDS-F | ATGTTTCCAAGCTATTATTC |
730-CDS-R | TTAGATTGAATAATTCCATA |
740-CDS-F | ATGAATATCTTTGAAACCTA |
740-CDS-R | TTAGATAGAATAATTCCATA |
750-CDS-F | ATGGATATCTTTCGAAGCTA |
750-CDS-R | TTAGATAGAATAGCTCCATA |
pRI101-720-F | agagttgttgattcagaattcATGATGAAGTTATTCTTAGCTTCAAATAAC |
pRI101-720-R | ctgtacaagcccgggggtaccTATAGAATAATTCCATAAAGGCATATAAGC |
pRI101-730-F | agagttgttgattcagaattcATGTTTCCAAGCTATTATTCGGAGC |
pRI101-730-R | ctgtacaagcccgggggtaccTTAGATTGAATAATTCCATAAAGACATGTAA |
pRI101-740-F | agagttgttgattcagaattcATGAATATCTTTGAAACCTATTATTCAGACC |
pRI101-740-R | ctgtacaagcccgggggtaccTTAGATAGAATAATTCCATAAAGTCATGTAAGC |
pRI101-750-F | agagttgttgattcagaattcATGGATATCTTTCGAAGCTATTATTCG |
pRI101-750-R | ctgtacaagcccgggggtaccTTAGATAGAATAGCTCCATAAAGGCATAT |
720qPCR-F | CCAACAGTAGAAATGGCAGCTAGAG |
720qPCR-R | CTTCCGACTGATCTGATGATGATGG |
730qPCR-F | GAACTTTGCTGACTCTGTTTGGAG |
730qPCR-R | CCTCATTCATAGAGAGGGCCTTTTC |
740qPCR-F | TAAAGATATTCAAAAGGCGGCCGC |
740qPCR-R | CTCTGGCGCTTCTTCGTTCATAAA |
750qPCR-F | GCTATAGCATTAAGAGGCCGTTCT |
750qPCR-R | CATCCGGAGTACTAGATTGATCTCC |
Actin-F | GGGATGGAGAAGTTTGGTGGTGG |
Actin-R | CTTCGACCAAGGGATGGTGTAGC |
Fig. 1 Sequence alignment (A) and evolutionary analysis (B) of OsDREB1C homologous protein in potato A: Amino acid sequence alignment of OsDREB1C homologous protein in potato. B: Evolutionary analysis of OsDREB1C homologous protein in potato
蛋白质 Protein | 氨基酸数量Amount of amino acids | 分子质量Molecular mass/Da | 原子总数 Total atoms | 不稳定系数Coefficient of instability | pI | 脂溶性指数Fat solubility index | 亲水指数Hydrophilic index | 亚细胞定位Subcellular localization |
---|---|---|---|---|---|---|---|---|
StDREB720 | 199 | 22 478 | 3 125 | 65.51 | 6.44 | 69.70 | -0.37 | 细胞核 |
StDREB730 | 236 | 26 319 | 3 628 | 61.92 | 4.98 | 67.50 | -0.48 | 细胞核、细胞质 |
StDREB740 | 258 | 28 601 | 3 909 | 70.35 | 4.55 | 57.67 | -0.56 | 细胞核、细胞质 |
StDREB750 | 217 | 24 264 | 3 334 | 55.26 | 5.20 | 57.65 | -0.60 | 细胞核、细胞质 |
Table 3 Physicochemical analysis of StDREBs protein
蛋白质 Protein | 氨基酸数量Amount of amino acids | 分子质量Molecular mass/Da | 原子总数 Total atoms | 不稳定系数Coefficient of instability | pI | 脂溶性指数Fat solubility index | 亲水指数Hydrophilic index | 亚细胞定位Subcellular localization |
---|---|---|---|---|---|---|---|---|
StDREB720 | 199 | 22 478 | 3 125 | 65.51 | 6.44 | 69.70 | -0.37 | 细胞核 |
StDREB730 | 236 | 26 319 | 3 628 | 61.92 | 4.98 | 67.50 | -0.48 | 细胞核、细胞质 |
StDREB740 | 258 | 28 601 | 3 909 | 70.35 | 4.55 | 57.67 | -0.56 | 细胞核、细胞质 |
StDREB750 | 217 | 24 264 | 3 334 | 55.26 | 5.20 | 57.65 | -0.60 | 细胞核、细胞质 |
蛋白质Protein | 无规则卷曲Random coil/% | α-螺旋 α-helix/% | 延伸链 Extended strand/% | β-转角 β-turn/% | 保守结构域Conserved domain(super family) | 保守结构域位置 Conservative domain location/aa |
---|---|---|---|---|---|---|
StDREB720 | 49.25 | 34.17 | 14.57 | 2.01 | AP2 | 28-86 |
StDREB730 | 53.39 | 31.36 | 11.86 | 3.39 | AP2 | 64-122 |
StDREB740 | 49.22 | 35.27 | 11.63 | 3.88 | AP2 | 51-111 |
StDREB750 | 58.99 | 26.73 | 11.06 | 3.23 | AP2 | 62-120 |
Table 4 Structure and secondary structure prediction of StDREBs protein
蛋白质Protein | 无规则卷曲Random coil/% | α-螺旋 α-helix/% | 延伸链 Extended strand/% | β-转角 β-turn/% | 保守结构域Conserved domain(super family) | 保守结构域位置 Conservative domain location/aa |
---|---|---|---|---|---|---|
StDREB720 | 49.25 | 34.17 | 14.57 | 2.01 | AP2 | 28-86 |
StDREB730 | 53.39 | 31.36 | 11.86 | 3.39 | AP2 | 64-122 |
StDREB740 | 49.22 | 35.27 | 11.63 | 3.88 | AP2 | 51-111 |
StDREB750 | 58.99 | 26.73 | 11.06 | 3.23 | AP2 | 62-120 |
名称Name | 序列Sequence | 数量Account | 功能Function |
---|---|---|---|
Circadian | CAAAGATATC | 720/1 740/3 750/1 | 参与昼夜节律控制的顺式作用调节元件Cis-acting regulatory element involved in circadian control |
WUN-motif | AAATTTCCT | 720/1 | 伤口敏感元件Wound-sensitive element |
CGTCA-motif | CGTCA | 720/2 730/1 | 参与水杨酸反应的顺式作用调节元件 Cis-acting regulatory element involved in the MeJA-responsiveness |
Box 4 | ATTAAT | 720/4 730/4 740/5 750/9 | 参与光响应的部分保守 DNA 模块 Part of a conserved DNA module involved in light-responsiveness |
MBS | CAACTG | 720/1 730/2 750/1 | 与干旱诱导有关的 MYB 结合位点MYB binding site involved in drought-inducibility |
TCT-motif | TCTTAC | 720/1 730/1 750/2 | 部分光响应元件Part of a light-responsive element |
G-box | CACGTG | 720/1 730/2 740/2 750/2 | 参与光反应的顺式作用调节元件Cis-acting regulatory element involved in light-responsiveness |
TATC-box | TATCCCA | 730/1 740/1 750/1 | 参与赤霉素反应的顺式作用元件Cis-acting element involved in gibberellin-responsiveness |
GT1-motif | GGTTAA | 720/1 730/5 740/1 | 光响应元件Light-responsive element |
LTR | CCGAAA | 730/4 | 参与低温响应的顺式作用元件Cis-acting element involved in low-temperature responsiveness |
ARE | AAACCA | 730/7 750/1 | 厌氧诱导必不可少的顺式作用的调节元件 Cis-acting regulatory element essential for the anaerobic induction |
AuxRR-core | GGTCCAT | 740/1 | 参与生长素反应的顺式作用调节元件 Cis-acting regulatory element involved in auxin-responsiveness |
TGA-element | AACGAC | 740/1 750/1 | 生长素响应元件Auxin-responsive element |
ABRE | CACGTG | 720/1 740/1 750/2 | 参与脱落酸反应的顺式作用元件Cis-acting element involved in the abscisic acid-responsiveness |
Table 5 Cis-acting elements in promoters of StDREBs gene
名称Name | 序列Sequence | 数量Account | 功能Function |
---|---|---|---|
Circadian | CAAAGATATC | 720/1 740/3 750/1 | 参与昼夜节律控制的顺式作用调节元件Cis-acting regulatory element involved in circadian control |
WUN-motif | AAATTTCCT | 720/1 | 伤口敏感元件Wound-sensitive element |
CGTCA-motif | CGTCA | 720/2 730/1 | 参与水杨酸反应的顺式作用调节元件 Cis-acting regulatory element involved in the MeJA-responsiveness |
Box 4 | ATTAAT | 720/4 730/4 740/5 750/9 | 参与光响应的部分保守 DNA 模块 Part of a conserved DNA module involved in light-responsiveness |
MBS | CAACTG | 720/1 730/2 750/1 | 与干旱诱导有关的 MYB 结合位点MYB binding site involved in drought-inducibility |
TCT-motif | TCTTAC | 720/1 730/1 750/2 | 部分光响应元件Part of a light-responsive element |
G-box | CACGTG | 720/1 730/2 740/2 750/2 | 参与光反应的顺式作用调节元件Cis-acting regulatory element involved in light-responsiveness |
TATC-box | TATCCCA | 730/1 740/1 750/1 | 参与赤霉素反应的顺式作用元件Cis-acting element involved in gibberellin-responsiveness |
GT1-motif | GGTTAA | 720/1 730/5 740/1 | 光响应元件Light-responsive element |
LTR | CCGAAA | 730/4 | 参与低温响应的顺式作用元件Cis-acting element involved in low-temperature responsiveness |
ARE | AAACCA | 730/7 750/1 | 厌氧诱导必不可少的顺式作用的调节元件 Cis-acting regulatory element essential for the anaerobic induction |
AuxRR-core | GGTCCAT | 740/1 | 参与生长素反应的顺式作用调节元件 Cis-acting regulatory element involved in auxin-responsiveness |
TGA-element | AACGAC | 740/1 750/1 | 生长素响应元件Auxin-responsive element |
ABRE | CACGTG | 720/1 740/1 750/2 | 参与脱落酸反应的顺式作用元件Cis-acting element involved in the abscisic acid-responsiveness |
Fig. 3 Expression pattern of StDREBs genes in potato The error bar indicates the standard deviation(n=3); significance analysis uses t test, * 0.05>P≥0.01; ** 0.01>P≥0.001; *** P<0.001; **** P≤0.0001, the same below
[1] | 王金秋, 武舜臣. 马铃薯主粮化战略的动力、障碍与前景[J]. 农业经济, 2018(4): 17-19. |
Wang JQ, Wu SC. Motivation, obstacles and prospects of potato staple food strategy[J]. Agric Econ, 2018(4): 17-19. | |
[2] | Zaheer K, Akhtar MH. Potato production, usage, and nutrition—a review[J]. Crit Rev Food Sci Nutr, 2016, 56(5): 711-721. |
[3] | 张小燕, 白鸥, 李少萍, 等. 北京大型超市马铃薯制品市场调查分析及对策建议[J]. 中国农业文摘-农业工程, 2022, 34(1): 80-84. |
Zhang XY, Bai O, Li SP, et al. Investigation and analysis of potato products market in large supermarkets in Beijing and countermeasures and suggestions[J]. Agric Sci Eng China, 2022, 34(1): 80-84. | |
[4] |
Beals KA. Potatoes, nutrition and health[J]. Am J Potato Res, 2019, 96(2): 102-110.
doi: 10.1007/s12230-018-09705-4 |
[5] | Gervais T, Creelman A, Li XQ, et al. Potato response to drought stress: physiological and growth basis[J]. Front Plant Sci, 2021, 12: 698060. |
[6] | 刘悦沁. 马铃薯主粮化及其绿色消费主张[J]. 中国果树, 2021(12): 后插6. |
Liu YQ. Potato staple food and its green consumption proposition[J]. China Fruits, 2021(12): 后插6. | |
[7] | 孙根紧, 王滨玥, 刘彦秀. 中国马铃薯种植区域比较优势的时空演进[J]. 西昌学院学报: 自然科学版, 2021, 35(1): 1-7, 11. |
Sun GJ, Wang BY, Liu YX. Research on spatial-temporal evolution of comparative advantages of potato planting regions in China[J]. J Xichang Univ Nat Sci Ed, 2021, 35(1): 1-7, 11. | |
[8] |
Ludemann CI, Gruere A, Heffer P, et al. Global data on fertilizer use by crop and by country[J]. Sci Data, 2022, 9(1): 501.
doi: 10.1038/s41597-022-01592-z pmid: 35978058 |
[9] | Pahalvi HN, Rafiya L, Rashid S, et al. Chemical fertilizers and their impact on soil health[M]// Microbiota and Biofertilizers, Vol 2. Cham: Springer, 2021: 1-20. |
[10] |
Yousaf M, Li JF, Lu JW, et al. Effects of fertilization on crop production and nutrient-supplying capacity under rice-oilseed rape rotation system[J]. Sci Rep, 2017, 7(1): 1270.
doi: 10.1038/s41598-017-01412-0 pmid: 28455510 |
[11] | 孟丽丽. 马铃薯块茎形成机制及其对品种与氮素的响应[D]. 呼和浩特: 内蒙古农业大学, 2020. |
Meng LL. Mechanism of potato tuber formation and its response to varieties and nitrogen[D]. Hohhot: Inner Mongolia Agricultural University, 2020. | |
[12] | Vitousek PM, Aber JD, Howarth RW, et al. Human alteration of the global nitrogen cycle: Sources and consequences[J]. Ecol Appl, 1997, 7(3): 737-750. |
[13] | 冯军, 郑彩霞. DREB转录因子在植物非生物胁迫中的作用及应用研究[J]. 植物生理学报, 2011, 47(5): 437-442. |
Feng J, Zheng CX. Research and application prospect of DREB transcription factor in plant abiotic stress resistance[J]. Plant Physiol J, 2011, 47(5): 437-442. | |
[14] | 李健, 王雅晴, 等. CBF转录因子在植物抗逆和生长发育中的重要功能[J]. 植物生理学报, 2017, 53(12): 2045-2056. |
Li J, Wang YQ, et al. The important function of CBF transcription factors in plant stress tolerance, growth and development[J]. Plant Physiol J, 2017, 53(12): 2045-2056. | |
[15] | 汪泽文, 杨依维, 王鹏飞, 等. 欧李DREB基因家族的鉴定与分析[J]. 植物生理学报, 2020, 56(3): 413-422. |
Wang ZW, Yang YW, Wang PF, et al. Identification and analysis of DREB gene family in Cerasus humilis[J]. China Ind Econ, 2020, 56(3): 413-422. | |
[16] | Zhang Y, Xia PG. The DREB transcription factor, a biomacromolecule, responds to abiotic stress by regulating the expression of stress-related genes[J]. Int J Biol Macromol, 2023, 243: 125231. |
[17] |
Akhtar M, Jaiswal A, Taj G, et al. DREB1/CBF transcription factors: their structure, function and role in abiotic stress tolerance in plants[J]. J Genet, 2012, 91(3): 385-395.
pmid: 23271026 |
[18] | Mei FM, Chen B, Du LY, et al. A gain-of-function allele of a DREB transcription factor gene ameliorates drought tolerance in wheat[J]. Plant Cell, 2022, 34(11): 4472-4494. |
[19] | Qian C, Li LL, Guo HH, et al. Genome-wide analysis of DREB family genes and characterization of cold stress responses in the woody plant Prunus nana[J]. Genes, 2023, 14(4): 811. |
[20] | Hassan S, Berk K, Aronsson H. Evolution and identification of DREB transcription factors in the wheat genome: modeling, docking and simulation of DREB proteins associated with salt stress[J]. J Biomol Struct Dyn, 2022, 40(16): 7191-7204. |
[21] | Rai KK, Rai N, Rai SP. Prediction and validation of DREB transcription factors for salt tolerance in Solanum lycopersicum L.: an integrated experimental and computational approach[J]. Environ Exp Bot, 2019, 165: 1-18. |
[22] | Wei SB, Li X, Lu ZF, et al. A transcriptional regulator that boosts grain yields and shortens the growth duration of rice[J]. Science, 2022, 377(6604): eabi8455. |
[23] | 郭荔雯, 摆福红, 沙晓东, 等. 番茄叶片及果实总RNA 提取方法的比较[J]. 湖北农业科学, 2021, 60(3): 141-144. |
Guo LW, Bai FH, Sha XD, et al. Comparison of extraction methods of total RNA from tomato leaves and fruits[J]. Hubei Agricultural Sciences, 2021, 60(3):141-144.. | |
[24] | Koua AP, Oyiga BC, Baig MM, et al. Breeding driven enrichment of genetic variation for key yield components and grain starch content under drought stress in winter wheat[J]. Front Plant Sci, 2021, 12: 684205. |
[25] |
韩芳英, 胡昕, 王楠楠, 等. DREBs响应植物非生物逆境胁迫研究进展[J]. 生物技术通报, 2023, 39(11): 86-98.
doi: 10.13560/j.cnki.biotech.bull.1985.2023-0124 |
Han FY, Hu X, Wang NN, et al. Research progress in response of DREBs to abiotic stress in plant[J]. Biotechnol Bull, 2023, 39(11): 86-98. | |
[26] | Kumar S, Muthuvel J, Sadhukhan A, et al. Enhanced osmotic adjustment, antioxidant defense, and photosynthesis efficiency under drought and heat stress of transgenic cowpea overexpressing an engineered DREB transcription factor[J]. Plant Physiol Biochem, 2022, 193: 1-13. |
[27] | Wang C, Han J, et al. Pan-genome-wide identification and transcriptome-wide analysis of DREB genes that respond to biotic and abiotic stresses in cucumber[J]. Agriculture, 2022, 12(11): 1879. |
[28] | Onele AO, Mazina AB, Leksin IY, et al. DsDBF1, a type A-5 DREB gene, identified and characterized in the moss Dicranum scoparium[J]. Life, 2022, 13(1): 90. |
[29] | 王雷立, 董柯清, 张严玲, 等. 玉米DREB转录因子家族的全基因组鉴定与分析[J]. 湖南农业大学学报: 自然科学版, 2022, 48(3): 270-281. |
Wang LL, Dong KQ, Zhang YL, et al. Genome-wide identification and analysis of DREB transcription factor family in maize[J]. J Hunan Agric Univ Nat Sci, 2022, 48(3): 270-281. |
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