Heterologous Expression and Characterization of Xylanase XYA6205 from Stachybotrys chartarum

Abstract

Abstract: Xylanase, the main enzyme to hydrolysis hemicellulose, has important application in industry. In this study, a gene xya6205 （with signal peptide）from Stachybotrys chartarum was inserted to an expression vector, eventually an recombinant plasmid pGm-xya6205 was constructed, and then transformed into A. niger G1 mediated by PEG4000. The transformants were confirmed by PCR analysis, and the recombinant xylanase of heterologously expressed protein in SDS-PAGE was about 20 kD. The optimal temperature and pH of the enzyme activity was 50℃ and 5.8, respectively. The recombinant enzyme specific activity reached 392 U/mg by DNS method at optimal condition. The recombinant enzyme remained 83% activity after 18 h in the alkaline buffer.

Key words: Xylanase, Aspergillus niger, Cloning, Activity, Stachybotrys chartarum

Wang Hongxia, Wang Huaming, Zhang Dalong, Luo Cheng. Heterologous Expression and Characterization of Xylanase XYA6205 from Stachybotrys chartarum[J]. Biotechnology Bulletin, 2013, 0(2): 130-134.

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