Molecular Cloning of Sugarcane ASR Gene（SoASR）and Its Expression Analysis

Abstract

Abstract: In this study, the full length of SoASR cDNA obtained by homologous cloning and RT-PCR. It consists of 753 bp with an open reading frame of 429 bp, encoding a polypeptide of 142 amino acids, and its GenBank accession number is JX470187. Homology analysis showed that SoASR were clustered into a group with barley, maize and sorghum with homology of 79%, 93% and 88%, respectively. A 32.0 kD heterologous protein was obtained when SoASR gene was expressed in E. coli. The results of quantitative real-time PCR analysis showed that, the mRNA of ASR was first up-regulated and then down-regulated in the sugarcane variety with strong cold resistance, GT28, and down-regulated in the sugarcane variety with weak cold resistance, YL6, under low temperature stress. The expressions of SoASR gene were induced in two varieties. These results suggested that SoASR might be involved in response to cold stress in sugarcane.

Key words: Sugarcane, ABA-/stress-/ripening-induced protein（ASR）, Clone Expression

Huang Xing, Yang Litao, Zhang Baoqing, Song Xiupeng, Li Yangrui, Wang Sheng. Molecular Cloning of Sugarcane ASR Gene（SoASR）and Its Expression Analysis[J]. Biotechnology Bulletin, 2013, 0(2): 93-99.

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