Cloning and Sequence Analysis of Actin Gene from Energy Plant Euphorbia lathyris

Abstract

Abstract: Actins exist widely in plants. In this study, homologous cloning and RACE technology were employed to obtain the Actin fulllength cDNA sequence of Euphorbia lathyris （named ElActin, GenBank accession No. KC182753）. Sequence analysis showed that ElActin contained a 1 134 bp open reading frame （ORF） encoding a 377 amino acid residues, a 5'-UTR of 247 bp and a 3'-UTR of 362 bp. Compared with other plants, the homology of nucleotide sequence and amino acid sequence is above 82% and 97%, respectively.

Key words: Euphorbia lathyris, Actin gene, Homologous cloning, RACE, Sequence analysis

Li Chunxia, Yao Zhengying, Zhang Weiming, Sun Lijun. Cloning and Sequence Analysis of Actin Gene from Energy Plant Euphorbia lathyris[J]. Biotechnology Bulletin, 2013, 0(3): 149-154.

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