Gold Immunochromatography Assay for Rapid Detection of Fluoroquinolones——Being Used with a Readout Instrument&#160;of Colloidal Gold Test Paper Card Together

Abstract

Abstract: To establish a rapid assay for detecting fluoroquinolones in milk, gold immunochromatography assay was studied, which was used with a readout instrument of colloidal gold test paper card together. The limit of detection(enrofloxacin, sarafloxaxcin, difloxacin, ofloxacin, norfloxacin, ciprofloxacin, pefloxacin, flumequine, danofloxacin)is 20 μg/L, and(enoxacin, oxolinic acid)is 40 μg/L. We can obtain the results in 10 minutes. The assay gives no false positive and false negative results. The method is accurate, simple, reliable and convenient to rapidly detect fluoroquinolones of a lot of samples on the spot.

Key words: Fluoroquinolones, Gold immunochromatography assay strip, Feadout instrument of colloidal gold test paper card, Rapid detection

Ma Yinsheng, Wan Yuping, Liu Qingjun, Jia Fangfang, Zhang Yu, Du Meihong . Gold Immunochromatography Assay for Rapid Detection of Fluoroquinolones——Being Used with a Readout Instrument of Colloidal Gold Test Paper Card Together[J]. Biotechnology Bulletin, 2013, 0(6): 226-231.

References

[1] 潘明飞, 王俊平, 王硕, 等. 食品中氟喹诺酮类药物残留色谱检测方法的研究[J]. 食品与机械, 2009, 25(5):129-132.
[2] 振华, 魏东, 张乃生. 氟喹诺酮类药物残留检测方法研究进展[J]. 动物医学进展, 2007, 28(3):98-101.
[3] 潘孝成, 祁克宗, 朱良强, 等. 氟喹诺酮类药物的多残留酶免疫分析研究[J]. 中国畜牧兽医, 2007, 34(11):126-128.
[4] 映梅, 张秀英, 张峻峰. 动物性食品中氟喹诺酮类药物的残留分析[J]. 中国兽药杂志, 2004, 38(8):29-32.
[5] 赖卫华, 熊勇华, 陈高明, 等. 应用胶体金试纸条快速检测赭曲霉毒素A的研究[J]. 食品科学, 2005, 26(5):204-207.
[6] 董志远, 赵晓凤, 宫秀杰, 等. 牛奶中氟喹诺酮类药物残留量检测方法研究[J]. 中国兽药杂志, 2008, 42(10):14-16.
[7] Hoof NV, Wasch KD, Okerman L, et al. Validation of a liquid chrom-atography-tandem mass spectrometric method for the quantification of eight quinolones in bovine muscle, milk and aquacultured products [J]. Analytica Chimica Acta, 2005(529):265-272.
[8] 饶勇, 曾振灵, 杨桂香, 等. 液相色谱-质谱联用检测牛奶中氟喹诺酮类药物残留的确证方法[J]. 中国农业科学, 2007, 40(5):1033-1041.
[9] 岳振峰, 谢丽琪, 陈小霞, 等. 牛奶中1 6种喹诺酮类药物残留量的高效液相色谱-串联质谱法测定[J]. 分析测试学报, 2008, 27(3):240-243.
[10] 叶鹿鸣, 谢东华. 高效液相色谱荧光检测法测定牛奶中3 种氟喹诺酮残留研究[J]. 中国卫生检验杂志, 2008, 18(5):804-806.
[11] 边永玲, 李秀娟, 郭建. 氟喹诺酮类兽药残留检测方法研究进展[J]. 中国农学通报, 2008, 24(6):28-31.
[12] 魏东, 刘英.氟喹诺酮类药物残留多元快速检测试纸条的研制[J].核农学报, 2012, 26(9):1278-1283.
[13] 冯婷婷,  刘一兵,  侯惠仁.氟喹诺酮类药物胶体金试纸的研制[J]. 中国畜牧兽医, 2012, 39(9):54-58.
[14] 胡珩. 氟喹诺酮多组分残留检测试纸条的研制[D].长春:吉林大学, 2008.
[15] 邓省亮, 赖卫华, 许杨. 胶体金免疫层析法快速检测黄曲霉毒素B1的研究[J]. 食品科学, 2007, 28(2):232-236.

Gold Immunochromatography Assay for Rapid Detection of Fluoroquinolones——Being Used with a Readout Instrument of Colloidal Gold Test Paper Card Together

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