Culture and Characterization of Chicken Embryonic Stem Cells in vitro

Abstract

Abstract: The blastoderm cells isolated from the stage X embryos of chicken were cultured on feeder layer of mouse embryonic fibroblasts（MEF）with high glucose DMEM supplemented with 10 ng/mL bFGF, 20 ng/mL hIGF-1, 2 ng/mL mSCF, 2 ng/mL hIL-11 and 1 000 U/mL LIF. Typical chicken embryonic stem cells（cESCs）colonies with high endogenous AKP activity were observed in the culture system. The cESCs derived from blastoderm cells expressed pluripotent markers SSEA-1 and Oct-4 as determined by immunocytological analysis. RT-PCR analysis further confirmed the expression of cENS-1, a gene specifically expressed in cESCs and early embryo. These results demonstrate the feasibility for using the culture system in isolating cESCs from the stage X chicken embryos and supporting the growth of cESCs with undifferentiating state in vitro.

Key words: Chicken, Embryonic stem cells, Blastodermal cells, In vitro culture, Characterization

He Wenjun,Liu Hong,Ye Lingling,Li Shichong,Wang Qiwei,Chen Zhaolie. Culture and Characterization of Chicken Embryonic Stem Cells in vitro[J]. Biotechnology Bulletin, 2013, 0(7): 94-98.

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