Separation of Plasma Membrane from Nasopharyngeal Carcinoma Cell Line 6-10B and Preliminary Analysis of Its Protein Profile

Abstract

Abstract: To establish plasma membrane（PM）protein profile of 6-10B nasopharyngeal carcinoma（NPC）cell, and accumulate valuable data for understanding the carcinogenesis mechanism of NPC. First of all, two-phase partition in combination with differential velocity centrifugation was used to purify PM of 6-10B cell. Then, purified PM were detected the purity by Western blot and electron microscopy. Next, SDS-PAGE was used to isolate PM proteins. After that, PM proteins were identified using mass spectrometry and bioinformatics. Finally, identified PM proteins were determined relevance with other tumors in human cancers database（dbDEPD）searching. A total of 406 proteins of 6-10B cell were identified, and 255 proteins were PM and membrane-associated proteins（accounting for 62.8%）. 46 kinds of proteins in 145 identified PM proteins were tumor-related proteins, of which 18 proteins associated with tumor metastasis. Further analysis found that CD225, CD104 and p63 were oral cancer（belong to head and neck cancer）metastasis related proteins, suggesting that they may be potential metastasis associated proteins in NPC. A PM protein profile of NPC 6-10B cell has been established, which provides useful information to investigate carcinogenesis mechanism of NPC.

Key words: Nasopharyngeal carcinoma, 6-10B cell, Plasma membrane, Proteomics, Aqueous two-phase partition, Mass spectrometry

Zhang Pengfei, Zeng Guqing^，, Tang Can’e, Yi Hong, Liang Songping, Chen Zhuchu, Xiao Zhiqiang. Separation of Plasma Membrane from Nasopharyngeal Carcinoma Cell Line 6-10B and Preliminary Analysis of Its Protein Profile[J]. Biotechnology Bulletin, 2013, 0(8): 176-183.

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