Abstract: Germination seeds were used to research the genetic transformation technology of Agrobacterium-mediated in soybean. The growing point, cotyledon node and hypocotyl of germination seed were used as explants, and the plasmid that carried with the resistance to the herbicide glyphosate gene EPSPS and glufosinate resistance screening gene Bar was transferred to soybean by Agrobacterium-mediated. Transformants were obtained by co-culture and potted plant growing. After the double PCR detection as well as the two herbicide resistance identification of transgenic T₀ and T₁ plants, the positive strains were acquired. The experiment results showed that two genes resistance to herbicides have been successfully transferred to soybean via germinated seeds by Agrobacterium-mediated transformation method, with the transformation rate of approximately 0.37%.