Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (10): 81-86.

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Identification of Rust Resistant Genes Based on SCAR Markers in Common Bean（Phaseolus vulgaris L.）

Wu Xingbo1,2,, Yue Huan1,2,, Hao Junjie2, Zhang Xiaoyan2, Li Hongwei2, Wang Zhenqing2, Lü Xianghua2,   

  1. （1. College of Horticulture and Landscape Architecture，Southwest University，Chongqing 400715 ；2. Qingdao Academy of Agriculture Science，Qingdao 266100）
  • Received:2013-05-02 Revised:2013-10-15 Online:2013-10-14 Published:2013-10-15

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Abstract:

Ten SCAR primer combinations（SK14 、SA14 、SI19 、SBC6 、SAD12 、SAE19 、UR11-GT2 、KB126 、SF10 and SBA8） of common bean rust resistant genes were used to check the genome DNA of 78 common bean accessions. The SCAR primer pairs, SA14 and KB126, amplified bands without difference among all the tested accessions ；SI19 and SAD12 were not amplified target bands at all ；SK14 marker appeared in 8 accessions, SBC6 marker in 23 accessions, SAE19 marker in 28 accessions, UR11-GT2 marker in 5 accessions, SF10 marker in 62 accessions, and SBA8 marker in 76 accessions. 2-5 SCAR markers appeared in 65 accessions respectively. The types of rust resistant genes in each of the 78 accessions have been identified, and we found common that several rust resistant genes aggregated in one common bean accession.

Key words: Common bean, SCAR, Rust, Resistance gene, Molecular marker